pAAV-CAG-tdTomato (codon diversified) in Mouse (AAV Retrograde)
Addgene #59462-AAVrg
- Data Submitted By
- Max Joffe and Jeff Conn
- Submission Date
- June 20, 2019
- Publication Date
- June 20, 2019 (modified May 16, 2023)
- Abstract
- Not Provided
Any further use of this image requires permission from the lab who submitted the data.
- Vector Used
-
pAAV-CAG-tdTomato (codon diversified)
Addgene #59462-AAVrg
Virus & Injection
Virus
- Virus Name
- pAAV-CAG-tdTomato (codon diversified)
- Serotype
- AAV Retrograde
- Source
- Addgene
- Catalog Number
- 59462-AAVrg
- Promoter
- CAG
- Cargo Type
- Control
- Injection Titer / Dose
- 1.00E+13 GC/mL
- Injection Volume
- 200 nL
- Injection Rate
- 100 nL/min
- Injector Material
- Not Provided
- Injection Site / Route
- Basolateral nucleus of the amygdala, NeuroNames ID: 246
Other Details
- Species
- Mouse
- Strain or Cell Line
- C57/BL6J
- Source
- Jackson Labs
- Stock Number
- JAX: 000664
- Age at Injection
- 4-5 weeks
- Time After Injection
- 1 week
- Detection Method
- Direct fluorescence
- Assay & Results
- Aiming to target pyramidal cells in the basolateral amygdala. We also targeted medium spiny neurons in the anterior nucleus accumbens, at the interface between the core and shell. The aim was to record from pyramidal cells in deep layer prelimbic prefrontal cortex, which we found with extensive tdTomato expression after this procedure. Neurons within the injection site as well as pyramidal cells throughout the prefrontal cortex.
- Functional Testing
- Not applicable
- Other Information
- Worked wonderfully.
- Tips & Comments
- No. Very straightforward.