pAAV-hSyn-DIO-hM3D(Gq)-mCherry in Mouse (AAV2)
Addgene #44361-AAV2
- Data Submitted By
- Yanghui Sheng and King-Wai Yau
- Submission Date
- June 19, 2019
- Publication Date
- June 19, 2019 (modified May 16, 2023)
- Abstract
- Not Provided
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- Vector Used
-
pAAV-hSyn-DIO-hM3D(Gq)-mCherry
Addgene #44361-AAV2
Virus & Injection
Virus
- Virus Name
- pAAV-hSyn-DIO-hM3D(Gq)-mCherry
- Serotype
- AAV2
- Source
- Addgene
- Catalog Number
- 44361-AAV2
- Promoter
- Synapsin
- Cargo Type
- Chemogenetics
- Injection Titer / Dose
- 5.00E+12 GC/ml
- Injection Volume
- 2 μl per eye
- Injection Rate
- Not Provided
- Injector Material
- Not Provided
- Injection Site / Route
- Retina, NeuroNames ID : 1862
- Additional Details
- Targeting retinal ganglion cells. Virus was injected into Opn4-Cre mice. Melanopsin-expressing intrinsically photosensitive retinal ganglion cells ended up expressing hM3D from viral infection
Other Details
- Species
- Mouse
- Strain or Cell Line
- C57/BL6J background (Opn4-Cre is a BAC-transgene that we inserted into the mouse genome so that Cre recombinase is expressed in Opn4-positive cells)
- Source
- Not provided
- Other Information
- Virus was injected into Opn4-Cre mice.
- Age at Injection
- 1-3 months
- Time After Injection
- 8 weeks
- Detection Method
- Direct fluorescence
- Assay & Results
- Melanopsin-expressing intrinsically photosensitive retinal ganglion cells ended up expressing hM3D from viral infection.