Anti-MAPT [AT8] in Immunohistochemistry (Human)
Addgene #216150
- Data Submitted By
- Zhuhao Wu and Wei Wang
- Lab Name
- Zhuhao Wu, Weill Cornell Medicine
- Submission Date
- October 09, 2024
- Publication Date
- October 18, 2024 (modified November 22, 2024)
- DOI
- https://doi.org/10.57733/addgene.go1pq7
- Abstract
- Experimental results for Anti-MAPT [AT8] (Addgene #216150) in an immunohistochemistry assay with human brain samples.
- Citation
-
Anti-MAPT [AT8] in Immunohistochemistry (Human). Wu Z, Wang W 2024. Addgene Report, https://doi.org/10.57733/addgene.go1pq7
![Brain sections from the frontal pole were labeled with Anti-MAPT [AT8] from Addgene (216150-rAb) or Invitrogen (MN1020). As expected, the labeling patterns in individuals with Alzheimer's or Hoarding disorder suggests MAPT expression while that of an individual with diabetes and cancer does not. Human brain tissue was analyzed from postmortem sources and is available thanks to the generosity of the tissue donors and their families through Neuropathology Brain Bank & Research CoRE at Mount Sinai.](https://datahub-media.addgene.org/report-images/go1pq7/AT8_IHC1.png)
Image attribution: Zhuhao Wu and Wei Wang, Weill Cornell Medicine
This report is made available under the Creative Commons Attribution 4.0 International License.
- Antibodies Used
-
Anti-MAPT [AT8]
Addgene #216150 -
HRP-conjugated AffiniPure goat anti-mouse IgG, Fcγ subclass 2a specific
Jackson ImmunoResearch Labs #115-035-206
Materials & Methods
Sample
- Species
- Human
- Cell / Tissue Type
- Brain, frontal pole
- Additional Information
- 70 µm thick section.
- Preparation
- Free-floating
Buffer
- Name
- PBS
Fixative
- Fixative
- Formaldehyde/Paraformaldehyde
Permeabilization / Delipidation
- Delipidation
- Yes
Blocking
- Agent
- PTxwH buffer
Primary Antibody
- Name
- Anti-MAPT [AT8]
- Source
- Addgene
- Catalog Number
- 216150
- Species
- Mouse
- Gene / Target
- MAPT
- Concentration
- 1 µg/mL
- Time
- Overnight
- Temperature
- 4 ˚C
Secondary Antibody
- Name
- HRP-conjugated AffiniPure goat anti-mouse IgG, Fcγ subclass 2a specific
- Source
- Jackson ImmunoResearch Labs
- Catalog Number
- 115-035-206
- Concentration
- 1 µg/mL
- Time
- Overnight
- Temperature
- Room Temperature
Additional Information
- Protocol
- Thick-Section Based Antibody Screening (Link opens in a new window)
- Controls
- The labeling pattern in individuals with Alzheimer's or Hoarding disorder (expect MAPT expression) were compared to an individual with cancer (don't expect MAPT expression). Labeling was compared to an alternative anti-MAPT antibody (Invitrogen #MN1020).
Results
- Result
- Pass (The antibody worked under these conditions)
- Describe Results
- Labeling pattern was equivalent to Invitrogen #MN1020 antibody.