Anti-Slo1/BKAlpha maxi-K+ channel [L6/23R] in Western Blot (Rat)
Addgene #209586
- Data Submitted By
- James Trimmer
- Lab Name
- James Trimmer, UC Davis
- Submission Date
- October 06, 2024
- Publication Date
- October 11, 2024 (modified October 16, 2024)
- Abstract
- Experimental results for Anti-Slo1/BKAlpha maxi-K+ channel (Addgene #209586) in a western blot assay with a rat brain lysate. The labeling by the Anti-Slo1/BKAlpha maxi-K+ channel recombinant antibody produced at Addgene (R) was compared to its hybridoma parent (H).
![Crude rat membranes immunoblotted against Anti-Slo1/BKAlpha maxi-K+ channel [L6/23R] from the Trimmer Lab at UC Davis (H) or recombinant monoclonal antibody produced at Addgene (R).](https://datahub-media.addgene.org/report-images/3t4dm8/L6_23.png)
Image attribution: James Trimmer, UC Davis
This report is made available under the Creative Commons Attribution 4.0 International License.
- Antibodies Used
-
Anti-Slo1/BKAlpha maxi-K+ channel [L6/23R]
Addgene #209586 -
Goat anti-mouse IgG2a
Jackson ImmunoResearch Labs #115-035-206
Materials & Methods
Sample
- Species
- Rat
- Cell / Tissue Type
- Brain
- Additional Information
- Rat brain lysates were boiled at 95 °C 5 min prior to loading.
Buffer
- Name
- TBS
- pH
- 7.5
Blocking
- Agent
- Milk
- Concentration
- 4% w/v
- Time
- 45 min
- Temperature
- Room Temperature
Primary Antibody
- Name
- Anti-Slo1/BKAlpha maxi-K+ channel [L6/23R]
- Source
- Addgene
- Catalog Number
- 209586
- Species
- Mouse
- Gene / Target
- Kcnma1
- Concentration
- 0.5 µg/mL
- Time
- 45 min
- Temperature
- Room Temperature
Secondary Antibody
- Name
- Goat anti-mouse IgG2a
- Source
- Jackson ImmunoResearch Labs
- Catalog Number
- 115-035-206
- Concentration
- 1:8,333
- Conjugate
- HRP
- Time
- 45 min
- Temperature
- Room Temperature
Additional Information
- Additional Information
- Total rat brain extract was reduced and separated by denaturing SDS-PAGE.
- Protocol
- Trimmer lab immunoblot labeling protocol (Link opens in a new window)
- Controls
- Compared labeling by Addgene-produced recombinant antibody (Addgene #209586) to the validated hybridoma parent.
- Detection Method
- ECL
- Predicted Molecular Weight
- 110–130 kDa
Results
- Result
- Pass (The antibody worked under these conditions)
- Describe Results
- Equivalent signal strength and labeling pattern between the UC Davis and Addgene-produced antibodies.