IHC for Anti-Glypican 4 (GPC4) [IPI-mGPC4.9]
Addgene #222516
- Data Submitted By
- James Trimmer
- Lab Name
- James Trimmer, UC Davis
- Submission Date
- October 29, 2024
- Publication Date
- November 18, 2024 (modified November 18, 2024)
- DOI
- https://doi.org/10.57733/addgene.0vnll9
- Abstract
- Experimental results for Anti-Glypican 4 (GPC4) [IPI-mGPC4.9](Addgene #222516) in an immunohistochemistry assay with a rat sample.
- Citation
-
IHC for Anti-Glypican 4 (GPC4) [IPI-mGPC4.9]. Trimmer J 2024. Addgene Report, https://doi.org/10.57733/addgene.0vnll9
![Free-floating rat brain sections were fixed and labeled with purified Anti-Glypican 4 (GPC4) [IPI-mGPC4.9] (Addgene #222516).](https://datahub-media.addgene.org/report-images/0vnll9/GPC4.9_IHC.png)
Image attribution: James Trimmer, Ph.D.
This report is made available under the Creative Commons Attribution 4.0 International License.
- Antibodies Used
-
Anti-Glypican 4 (GPC4) [IPI-mGPC4.9]
Addgene #222516 -
Biotinylated goat anti-rabbit IgG (H+L)
Vector Labs #BA-1000
Materials & Methods
Sample
- Species
- Rat
- Cell / Tissue Type
- Brain
- Preparation
- Free-floating
Fixative
- Fixative
- Formaldehyde/Paraformaldehyde
Permeabilization / Delipidation
- Agent
- PBS containing 1:2000 avidin and 0.5% Triton X-100
- Time
- 45 min
- Temperature
- Room Temperature
Blocking
- Agent
- Serum
- Concentration
- 0.5%
- Time
- 45 min
- Temperature
- Room Temperature
Primary Antibody
- Name
- Anti-Glypican 4 (GPC4) [IPI-mGPC4.9]
- Source
- Addgene
- Catalog Number
- 222516
- Species
- Rabbit
- Gene / Target
- GPC4
- Concentration
- 1 µg/mL
- Time
- overnight
- Temperature
- 4 ˚C
Secondary Antibody
- Name
- Biotinylated goat anti-rabbit IgG (H+L)
- Source
- Vector Labs
- Catalog Number
- BA-1000
- Concentration
- 1 µg/mL
- Time
- 60 min
- Temperature
- Room Temperature
Additional Information
- Additional Information
- Validated by comparing to expression levels from In situ hybridization done by Allen Brain Atlas (Link opens in a new window) from a sagittal section of P56 mouse brain.
- Protocol
- Trimmer lab immunohistochemistry protocol (Link opens in a new window)
Results
- Result
- Pass (The antibody worked under these conditions)
- Describe Results
- Recognizes GPC4