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pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40 in Mouse (AAV1)

Addgene #100853-AAV1

Data Submitted By
Farhan Ali and Alex Kwan
Submission Date
July 10, 2019
Publication Date
July 10, 2019 (modified May 16, 2023)
Abstract
Not Provided
In vivo two-photon imaging at 1020 nm for excitation and ~610 nm for emission. PV-expressing GABAergic interneurons in the mouse neocortex, specifically dorsal anterior cingulate cortex/medial secondary motor cortex.
Caption: In vivo two-photon imaging at 1020 nm for excitation and ~610 nm for emission. PV-expressing GABAergic interneurons in the mouse neocortex, specifically dorsal anterior cingulate cortex/medial secondary motor cortex.

Any further use of this image requires permission from the lab who submitted the data.

Vector Used
pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40
Addgene #100853-AAV1

Virus & Injection

Virus

Virus Name
pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40
Serotype
AAV1
Source
Addgene
Catalog Number
100853-AAV1
Promoter
Synapsin
Cargo Type
Biosensor
Injection Titer / Dose
1.00E+13 GC/ml
Injection Volume
180 nL
Injection Rate
40 nL/min
Injector Material
Not Provided
Injection Site / Route
Dorsal part of the anterior cingulate area, NeuroNames ID: 1062
Additional Details
Injection site: dorsal anterior cingulate cortex/medial secondary motor cortex of mouse, superficial layers.
View in Addgene Catalog

Other Details

Species
Mouse
Strain or Cell Line
BL6* (see below)
Source
Jackson Labs
Other Information
Cross between 5xFAD (JAX Stock Number 006554) and PV-Cre animal (JAX 008069), with Cre expressed in PV neurons.
Age at Injection
8-24 weeks
Time After Injection
5 weeks
Detection Method
Direct fluorescence
Assay & Results
Cell type: parvalbumin-expressing GABAergic neurons.