pAAV.Syn.NES-jRGECO1a.WPRE.SV40 in Mouse (AAV1)
Addgene #100854-AAV1
- Data Submitted By
- Sindhuja Baskar and David Margolis
- Submission Date
- October 24, 2019
- Publication Date
- October 24, 2019 (modified May 16, 2023)
- Abstract
- Not Provided
Any further use of this image requires permission from the lab who submitted the data.
- Vectors Used
-
pAAV.Syn.NES-jRGECO1a.WPRE.SV40
Addgene #100854-AAV1 -
pGP-AAV-syn-jGCaMP7f-WPRE
Addgene #104488-AAV1 -
pAAV-CaMKIIa-hChR2(H134R)-EYFP
Addgene #26969-AAV1
Virus & Injection
Virus 1
- Virus Name
- pAAV.Syn.NES-jRGECO1a.WPRE.SV40
- Serotype
- AAV1
- Source
- Addgene
- Catalog Number
- 100854-AAV1
- Promoter
- Synapsin
- Cargo Type
- Biosensor
- Injection Titer / Dose
- 2.40E+13 GC/mL
- Injection Volume
- ~210-420 nL
- Injection Rate
- 42-84 nL/min
- Injector Material
- Not Provided
- Injection Site / Route
- Primary motor cortex, NeuroNames ID: 1910
- Additional Details
- Injection site: whisker M1.
Virus 2
- Virus Name
- pGP-AAV-syn-jGCaMP7f-WPRE
- Serotype
- AAV1
- Source
- Addgene
- Catalog Number
- 104488-AAV1
- Promoter
- Synapsin
- Cargo Type
- Biosensor
- Injection Titer / Dose
- 2.20E+13 GC/mL
- Injection Volume
- ~210-420 nL
- Injection Rate
- 42-84 nL/min
- Injector Material
- Not Provided
- Injection Site / Route
- Primary motor cortex, NeuroNames ID: 1910. Primary somatosensory cortex, NeuroNames ID: 1350. Posteromedian thalamic nucleus, NeuroNames ID: 1106.
- Additional Details
- Injection site: whisker M1. Injection site: whisker M1. Where: primary motor cortex (M1), primary somatosensory cortex (S1), striatum, posterior medial nucleus of the thalamus (POm).
Virus 3
- Virus Name
- pAAV-CaMKIIa-hChR2(H134R)-EYFP
- Serotype
- AAV1
- Source
- Addgene
- Catalog Number
- 26969-AAV1
- Promoter
- CaMKII
- Cargo Type
- Optogenetics
- Injection Titer / Dose
- 1.00E+13 GC/mL
- Injection Volume
- ~210 nL
- Injection Rate
- 42 nL/min
- Injector Material
- Not Provided
- Injection Site / Route
- Posteromedian thalamic nucleus, NeuroNames ID: 1106
- Additional Details
- Injection site: whisker M1. Where: primary motor cortex (M1), primary somatosensory cortex (S1), striatum, posterior medial nucleus of the thalamus (POm).
Other Details
- Species
- Mouse
- Strain or Cell Line
- C57Bl/6J, B6. Tg(Gpr26-cre)KO250
- Source
- Jackson Labs
- Stock Number
- 000664
- Age at Injection
- 49-78 days
- Time After Injection
- 3 weeks
- Detection Method
- Fiber Photometry
- Assay & Results
- Not Provided