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pAAV.CAG.Flex.GCaMP6s.WPRE.SV40 in Rat (AAV5)

Addgene #100842-AAV5

Data Submitted By
Erin Glennon and Robert Froemke
Submission Date
July 09, 2019
Publication Date
July 09, 2019 (modified May 16, 2023)
Abstract
Not Provided
Caption: Blue - DAPI Red - TH Cre Green - GFP objective: 10X microscope: Zeiss 700 confocal
Image attribution: Jasmin Multani

Any further use of this image requires permission from the lab who submitted the data.

Vector Used
pAAV.CAG.Flex.GCaMP6s.WPRE.SV40
Addgene #100842-AAV5

Virus & Injection

Virus

Virus Name
pAAV.CAG.Flex.GCaMP6s.WPRE.SV40
Serotype
AAV5
Source
Addgene
Catalog Number
100842-AAV5
Promoter
CAG
Cargo Type
Biosensor
Injection Titer / Dose
3.20E+13 GC/mL
Injection Volume
two 1 μL injections
Injection Rate
75 nL/min
Injector Material
Not Provided
Injection Site / Route
Locus ceruleus, NeuroNames ID: 583
View in Addgene Catalog

Other Details

Species
Rat
Strain or Cell Line
LE-Tg(TH-Cre)3.1Deis
Source
Rat Resource & Research Center
Stock Number
659
Age at Injection
3-4 months
Time After Injection
Tested 1 week to 8 weeks after injection
Detection Method
Immunohistochemistry, fiber photometry
Assay & Results
Noradrenergic cells of the locus coeruleus. Limited expression in noradrenergic cells of the locus coeruleus.
Functional Testing
I was able to get somewhat reliable fiber photometry signal.
Other Information
No
Tips & Comments
I tried multiple capsids and promoters and found that this combination is the best.