CN1390 Characterization
Addgene #163505
- Data Submitted By
- Boaz Levi , Jonathan Ting and Bosiljka Tasic
- Submission Date
- June 16, 2023
- Publication Date
- June 27, 2023 (modified June 27, 2023)
- DOI
- https://doi.org/10.57733/addgene.xwaevx
- Funding
- NIA/NIMH RF1MH114126, UF1MH128339
- Abstract
- Forebrain GABAergic cells are labeled in mouse brain by transduction with CN1390 AAV of the PHP.eB serotype. It was injected at a concentration of 5.0E+11 viral genomes per animal into month-old C57BL/6J mice via the retro-orbital route. The animal was perfused and the brain fixed in 4% paraformaldehyde four weeks post injection. The fixed brain tissue was sectioned, slide mounted, and imaged for native SYFP2 fluorescence. CN1390 has been extensively characterized and shown to label forebrain GABAergic cells in Mich et. al. (Cell Reports) 2021. https://doi.org/10.1016/j.celrep.2021.108754.
- Citation
-
CN1390 Characterization. Levi B, Ting J, Tasic B 2023. Addgene Report, https://doi.org/10.57733/addgene.xwaevx
Image attribution: VGT Pipeline, Allen Institute for Brain Science
This report is made available under the Creative Commons Attribution 4.0 International License.
- Vector Used
-
CN1390-rAAV-DLX2.0-minBG-SYFP2-WPRE3-BGHpA
Addgene #163505
Virus & Injection
Virus
- Virus Name
- CN1390-rAAV-DLX2.0-minBG-SYFP2-WPRE3-BGHpA
- Serotype
- PHP.eB
- Source
- Addgene
- Catalog Number
- 163505
- Virus Lot Number
- v163948G
- Promoter
- Other
- Other Promoter
- DLX2.0
- Cargo Type
- Control
- Injection Titer / Dose
- 5.0 x10e11 vg/animal
- Injection Volume
- 90 µl
- Injection Rate
- N/A
- Injector Material
- 31g insulin syringe
- Tip Diameter
- 31
- Coatings
- N/A
- Injection Time
- N/A
- Injection Site / Route
- Retro-orbital injection
- Additional Details
- AAV-PHP.eB/CN1390-rAAV-DLX2.0-minBG-SYFP2-WPRE3-BGHpAwas injected at a concentration of 5.0 x 10e11 viral genomes per animal into month-old C57BL/6J mice via the retro-orbital route.
Other Details
- Species
- Mouse
- Strain or Cell Line
- C57BL/6J
- Source
- Jackson Laboratory
- Stock Number
- 000664
- Age at Injection
- 1 month
- Sex
- Male
- Time After Injection
- 4 weeks
- Detection Method
- Native Fluorescence
- Assay & Results
- The animal was perfused and the brain fixed with 4% paraformaldehyde four weeks post injection. The fixed brain tissue was then sectioned, slide mounted, and imaged for native SYFP2 fluorescence on a Leica Aperio Versa slide scanner.