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CN1390 Characterization

Addgene #163505

Data Submitted By
Boaz Levi , Jonathan Ting and Bosiljka Tasic
Submission Date
June 16, 2023
Publication Date
June 27, 2023 (modified June 27, 2023)
DOI
https://doi.org/10.57733/addgene.xwaevx
Funding
NIA/NIMH RF1MH114126, UF1MH128339
Abstract
Forebrain GABAergic cells are labeled in mouse brain by transduction with CN1390 AAV of the PHP.eB serotype. It was injected at a concentration of 5.0E+11 viral genomes per animal into month-old C57BL/6J mice via the retro-orbital route. The animal was perfused and the brain fixed in 4% paraformaldehyde four weeks post injection. The fixed brain tissue was sectioned, slide mounted, and imaged for native SYFP2 fluorescence. CN1390 has been extensively characterized and shown to label forebrain GABAergic cells in Mich et. al. (Cell Reports) 2021. https://doi.org/10.1016/j.celrep.2021.108754.
Citation
CN1390 Characterization. Levi B, Ting J, Tasic B 2023. Addgene Report, https://doi.org/10.57733/addgene.xwaevx
Caption: A sagittal section of a whole mouse brain is shown on the left, and a portion of the cortex is shown on the right (including VISp and PFLp, cortical areas marked by yellow box). Forebrain GABAergic cells are labeled in mouse brain by transduction with AAV-PhP.eB/CN1390-rAAV-DLX2.0-minBG-SYFP2-WPRE3-BGHpA. CN1390 has been extensively characterized and shown to label forebrain GABAergic cells in Mich et. al. (Cell Reports) 2021. https://doi.org/10.1016/j.celrep.2021.108754.
Image attribution: VGT Pipeline, Allen Institute for Brain Science

This report is made available under the Creative Commons Attribution 4.0 International License.

Vector Used
CN1390-rAAV-DLX2.0-minBG-SYFP2-WPRE3-BGHpA
Addgene #163505

Virus & Injection

Virus

Virus Name
CN1390-rAAV-DLX2.0-minBG-SYFP2-WPRE3-BGHpA
Serotype
PHP.eB
Source
Addgene
Catalog Number
163505
Virus Lot Number
v163948G
Promoter
Other
Other Promoter
DLX2.0
Cargo Type
Control
Injection Titer / Dose
5.0 x10e11 vg/animal
Injection Volume
90 ┬Ál
Injection Rate
N/A
Injector Material
31g insulin syringe
Tip Diameter
31
Coatings
N/A
Injection Time
N/A
Injection Site / Route
Retro-orbital injection
Additional Details
AAV-PHP.eB/CN1390-rAAV-DLX2.0-minBG-SYFP2-WPRE3-BGHpAwas injected at a concentration of 5.0 x 10e11 viral genomes per animal into month-old C57BL/6J mice via the retro-orbital route.
View in Addgene Catalog

Other Details

Species
Mouse
Strain or Cell Line
C57BL/6J
Source
Jackson Laboratory
Stock Number
000664
Age at Injection
1 month
Sex
Male
Time After Injection
4 weeks
Detection Method
Native Fluorescence
Assay & Results
The animal was perfused and the brain fixed with 4% paraformaldehyde four weeks post injection. The fixed brain tissue was then sectioned, slide mounted, and imaged for native SYFP2 fluorescence on a Leica Aperio Versa slide scanner.