pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA in Mouse (AAV1)
Addgene #50943-AAV1
- Data Submitted By
- Katherine Sadleir and Robert Vassar
- Submission Date
- July 11, 2019
- Publication Date
- July 11, 2019 (modified May 16, 2023)
- Abstract
- Not provided
Any further use of this image requires permission from the lab who submitted the data.
- Vector Used
-
pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA
Addgene #50943-AAV1
Virus & Injection
Virus
- Virus Name
- pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA
- Serotype
- AAV1
- Source
- Addgene
- Catalog Number
- 50943-AAV1
- Promoter
- Synapsin
- Cargo Type
- Biosensor
- Injection Titer / Dose
- 1.00E+10 GC/ml
- Injection Volume
- 4 μl (2 μl per hemisphere)
- Injection Rate
- Injections were done by hand
- Injector Material
- Not provided
- Injection Site / Route
- Intracerebroventricular (ICV). NeuroNames ID : 2497
- Additional Details
- Neurons were targeted; We target the ventricles for widepsread transduction; Neurons in forebrain- cortex, hippocampus, some mid brain
Other Details
- Species
- Mouse
- Strain or Cell Line
- B6SJL F1 hybrid
- Source
- Not provided
- Stock Number
- 100012
- Age at Injection
- P0 (first 24 hrs after birth)
- Time After Injection
- 1 month to 6 months
- Detection Method
- Immunohistochemistry, Direct fluorescence
- Assay & Results
- Not provided
- Functional Testing
- The GCaMP6 activated spontaneously in some neurons, suggesting it worked effectively. The mRuby was visible in cell bodies and neurites, marking transduced cells
- Other Information
- No evidence of toxicity or other adverse effects. Expression persisted through life of animal, up to at least 16 months