pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3 in Mouse (AAV9)
Addgene #108912-AAV9
- Data Submitted By
- Julie Haas
- Submission Date
- July 17, 2020
- Publication Date
- July 17, 2020 (modified May 16, 2023)
- Abstract
- Not provided.
Image attribution: Atehsa Sahagun, Kevin Bender
Any further use of this image requires permission from the lab who submitted the data.
- Vector Used
-
pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3
Addgene #108912-AAV9
Virus & Injection
Virus
- Virus Name
- pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3
- Serotype
- AAV9
- Source
- Addgene
- Catalog Number
- 108912-AAV9
- Promoter
- CAG
- Cargo Type
- Optogenetics
- Injection Titer / Dose
- 1.00E+13 GC/ml
- Injection Volume
- two 200 nl injections
- Injection Rate
- 100 nl/min
- Injector Material
- Not provided
- Injection Site / Route
- Reticular nucleus of the thalamus NeuroNames ID: 365
- Additional Details
- Thalamic reticular nucleus - GABAergic neurons; Forebrain: Thalamus; TRN neurons.
Other Details
- Species
- Mouse
- Strain or Cell Line
- PV-cre heterozygote
- Source
- Not provided
- Age at Injection
- P28
- Time After Injection
- 3 weeks
- Detection Method
- Direct fluorescence, Whole-cell patch clamp
- Assay & Results
- Not provided
- Functional Testing
- ChroME, 1040 nm light used to drive spiking
- Other Information
- At 9 weeks* of expression, mRuby was widely diffuse, not nuclear. *Opportunity provided by covid-19