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pAAV-hSyn-DIO-mCherry in Mouse (AAV8)

Addgene #50459-AAV8

Data Submitted By: Avishek Adhikari
Submission Date: July 09, 2019
Publication Date: July 09, 2019 (modified May 16, 2023)
Abstract: Not Provided

mCherry expression in hypothalamus. Cre came from a transgenic mouse line that expressed cre in cck+ (cholecystokinin) cells. — Caption: mCherry expression in hypothalamus. Cre came from a transgenic mouse line that expressed cre in cck+ (cholecystokinin) cells.

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Vector Used: pAAV-hSyn-DIO-mCherry
Addgene #50459-AAV8

Virus & Injection

Virus

Virus Name: pAAV-hSyn-DIO-mCherry
Serotype: AAV8
Source: Addgene
Catalog Number: 50459-AAV8
Promoter: Synapsin
Cargo Type: Control
Injection Titer / Dose: 5.00E+12 GC/mL
Injection Volume: 60 nL
Injection Rate: 50 nL/min
Injector Material: Not Provided
Injection Site / Route: Hypothalamus, NeuroNames ID: 375
Additional Details: We targeted cholecystokinin expressing cells in the hypothalamus.

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Other Details

Species: Mouse
Strain or Cell Line: C57/BL6J (Ccktm1.1(cre)Zjh/J)
Source: Jackson Labs
Stock Number: 012706
Age at Injection: 2 months
Time After Injection: 2 weeks
Detection Method: Direct fluorescence
Assay & Results: Cre positive (cck-expressing) cells in the hypothalamus.