pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA in Mouse (AAV9)
Addgene #20298-AAV9
- Data Submitted By
- Liang Chen and Shaoyu Ge
- Submission Date
- June 19, 2019
- Publication Date
- June 19, 2019 (modified May 16, 2023)
- Abstract
- Not Provided
Image attribution: Liang
Any further use of this image requires permission from the lab who submitted the data.
- Vector Used
-
pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA
Addgene #20298-AAV9
Virus & Injection
Virus
- Virus Name
- pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA
- Serotype
- AAV9
- Source
- Addgene
- Catalog Number
- 20298-AAV9
- Promoter
- EF1a
- Cargo Type
- Optogenetics
- Injection Titer / Dose
- 1.00E+13 GC/ml
- Injection Volume
- 300 nl
- Injection Rate
- 100 nL/min
- Injector Material
- Not Provided
- Injection Site / Route
- Zona incerta, NeuroNames ID: 438
- Additional Details
- We want to target ZI PV cells.
Other Details
- Species
- Mouse
- Strain or Cell Line
- B6 PVcre
- Source
- Jackson Labs
- Stock Number
- JAX: 017320
- Age at Injection
- 8 weeks
- Time After Injection
- 3 weeks
- Detection Method
- Direct fluorescence
- Assay & Results
- We can see few glia cells surrounding the injection site.
- Functional Testing
- ChR2, we want to use light to activation special cells.
- Tips & Comments
- Do not dilute the virus.