AAV pmSyn1-EBFP-Cre in Mouse (AAV1)
Addgene #51507-AAV1
- Data Submitted By
- Henry Hallock and Keri Martinowich
- Submission Date
- July 02, 2019
- Publication Date
- July 02, 2019 (modified May 16, 2023)
- Abstract
- Not provided
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- Vector Used
-
AAV pmSyn1-EBFP-Cre
Addgene #51507-AAV1
Virus & Injection
Virus
- Virus Name
- AAV pmSyn1-EBFP-Cre
- Serotype
- AAV1
- Source
- Addgene
- Catalog Number
- 51507-AAV1
- Promoter
- Synapsin
- Cargo Type
- Recombinase
- Injection Titer / Dose
- 4.50E+12 GC/ml
- Injection Volume
- 600 nl
- Injection Rate
- 200 nl/min
- Injector Material
- Not provided
- Injection Site / Route
- Hippocampus, NeuroNames ID: 3157
- Additional Details
- Injecting into ventral hippocampus, targeting all cell types. Mouse used was RiboTag-HA mouse, so Cre was used to facilitate HA expression in all hippocampal neurons; Forebrain: Hippocampus; All cells in the ventral hippocampus
Other Details
- Species
- Mouse
- Strain or Cell Line
- C57/BL6J
- Stock Number
- JAX: 011029
- Age at Injection
- 3 months
- Time After Injection
- 4 weeks
- Detection Method
- Immunohistochemistry
- Functional Testing
- No tools were used
- Other Information
- No toxicity, no inflammation
- Tips & Comments
- The virus for this experiment worked very well