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pAAV-CaMKIIa-hChR2(H134R)-EYFP in Mouse (AAV1)

Addgene #26969-AAV1

Data Submitted By
Dong Wang
Submission Date
June 26, 2019
Publication Date
June 26, 2019 (modified May 16, 2023)
Abstract
Not Provided
confocal image taken with Leica TCS SP8 VIS/405. Fluorescenced in amygdala (projection site). The track of an optic fiber implantation into the basolateral amygdala is visible.
Caption: confocal image taken with Leica TCS SP8 VIS/405. Fluorescenced in amygdala (projection site). The track of an optic fiber implantation into the basolateral amygdala is visible.
Image attribution: Jun Liu
confocal imaging with Leica TCS SP8 VIS/405. Fluorescence in the ventral hippocampus (the virus partially spread to nearby cortex).
Caption: confocal imaging with Leica TCS SP8 VIS/405. Fluorescence in the ventral hippocampus (the virus partially spread to nearby cortex).
Image attribution: Jun Liu

Any further use of this image requires permission from the lab who submitted the data.

Vector Used
pAAV-CaMKIIa-hChR2(H134R)-EYFP
Addgene #26969-AAV1

Virus & Injection

Virus

Virus Name
pAAV-CaMKIIa-hChR2(H134R)-EYFP
Serotype
AAV1
Source
Addgene
Catalog Number
26969-AAV1
Promoter
CaMKII
Cargo Type
Optogenetics
Injection Titer / Dose
1.00E+13 GC/ml
Injection Volume
200 nl
Injection Rate
50 nl/min
Injector Material
Not Provided
Injection Site / Route
CA1 field, NeuroNames ID: 183
Additional Details
AAV was injected mainly into the ventral hippocampal CA1.
View in Addgene Catalog

Other Details

Species
Mouse
Strain or Cell Line
C57/BL6J
Source
Not provided
Age at Injection
10-13 weeks
Time After Injection
4 weeks
Detection Method
Direct fluorescence
Assay & Results
Fluorescence in the ventral hippocampus (the virus partially spread to nearby cortex) and in amygdala (projection site).
Functional Testing
Yes, in vivo recording in the basolateral amygdala confirmed that the ChR2 worked as expected
Tips & Comments
Inject less volume, such as 100 nl