pAAV-Ef1a-DIO ChETA-EYFP in Rat (AAV5)
Addgene #26968-AAV5
- Data Submitted By
- Erin Glennon and Robert Froemke
- Submission Date
- July 09, 2019
- Publication Date
- July 09, 2019 (modified May 16, 2023)
- Abstract
- Not Provided
Image attribution: Jasmin Multani
Any further use of this image requires permission from the lab who submitted the data.
- Vector Used
-
pAAV-Ef1a-DIO ChETA-EYFP
Addgene #26968-AAV5
Virus & Injection
Virus
- Virus Name
- pAAV-Ef1a-DIO ChETA-EYFP
- Serotype
- AAV5
- Source
- Addgene
- Catalog Number
- 26968-AAV5
- Promoter
- EF1a
- Cargo Type
- Optogenetics
- Injection Titer / Dose
- 1.90E+13 GC/mL
- Injection Volume
- two 1 μL injections
- Injection Rate
- 75 nL/min
- Injector Material
- Not Provided
- Injection Site / Route
- Locus ceruleus, NeuroNames ID: 583
Other Details
- Species
- Rat
- Strain or Cell Line
- LE-Tg(TH-Cre)3.1Deis
- Source
- Rat Resource & Research Center
- Stock Number
- 659
- Age at Injection
- 3-4 months
- Time After Injection
- 2 weeks
- Detection Method
- Immunohistochemistry
- Assay & Results
- Noradrenergic cells of the locus coeruleus.
- Functional Testing
- Yes, in one animal cortical desynchronization was confirmed upon light stimulation Parameters: 500 ms, 10 Hz, 1-3 mW, 10 ms pulse duration of blue light stimulation.