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pZac2.1 gfaABC1D-lck-GCaMP6f in Mouse (AAV5)

Addgene #52924-AAV5

Data Submitted By
Kira Poskanzer
Submission Date
July 29, 2019
Publication Date
July 29, 2019 (modified May 16, 2023)
Abstract
Not Provided
Caption: Acute cortical slice in V1 showing GCaMP+ astrocytes
Image attribution: Michelle Cahill

Any further use of this image requires permission from the lab who submitted the data.

Vector Used
pZac2.1 gfaABC1D-lck-GCaMP6f
Addgene #52924-AAV5

Virus & Injection

Virus

Virus Name
pZac2.1 gfaABC1D-lck-GCaMP6f
Serotype
AAV5
Source
Addgene
Catalog Number
52924-AAV5
Promoter
GFAP
Cargo Type
Biosensor
Injection Titer / Dose
4.92E+13 GC/mL
Injection Volume
30 nL/site, 8 sites per animal, 240 nL total
Injection Rate
180 nL/min
Injector Material
Not Provided
Injection Site / Route
Primary visual cortex, NeuroNames ID: 2102
Additional Details
Targeted astrocytes in V1.
View in Addgene Catalog

Other Details

Species
Mouse
Strain or Cell Line
C57/BL6J
Source
Not provided
Age at Injection
P0–3
Time After Injection
3 weeks
Detection Method
Direct fluorescence
Assay & Results
Targeted astrocytes in V1.
Functional Testing
Calcium dynamics looked as expected.