pAAV-Ef1a-DIO EYFP in Mouse (AAV9)
Addgene #27056-AAV9
- Data Submitted By
- Gaqi Tu and Kaori Takehara-Nishiuchi
- Submission Date
- June 23, 2019
- Publication Date
- June 23, 2019 (modified May 16, 2023)
- Abstract
- Not Provided
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- Vector Used
-
pAAV-Ef1a-DIO EYFP
Addgene #27056-AAV9
Virus & Injection
Virus
- Virus Name
- pAAV-Ef1a-DIO EYFP
- Serotype
- AAV9
- Source
- Addgene
- Catalog Number
- 27056-AAV9
- Promoter
- EF1a
- Cargo Type
- Control
- Injection Titer / Dose
- 3.30E+13 GC/mL
- Injection Volume
- 600-800 nL
- Injection Rate
- 200 nL for 100 nL and then 50 nL/min for 500~700 nL
- Injector Material
- Not Provided
- Injection Site / Route
- Basal forebrain, NeuroNames ID: 1997
Other Details
- Species
- Mouse
- Strain or Cell Line
- B6J.129S6-Chat/MwarJ
- Source
- Jackson Labs
- Stock Number
- 28861
- Other Information
- Homozygous for Chat.
- Age at Injection
- 8-12 weeks
- Time After Injection
- 6 weeks
- Detection Method
- Immunohistochemistry
- Assay & Results
- Basal forebrain, cholinergic neurons and non-cholinergic neurons (less than 2%). EYFP-green Cholinergic neurons were visualized using anti-ChAT antibody.
- Functional Testing
- The blue laser was used (Control group).