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AAV pmSyn1-EBFP-Cre in Mouse (AAV Retrograde)

Addgene #51507-AAVrg

Data Submitted By
Masashi Kondo and Masanori Mastuzaki
Submission Date
June 21, 2019
Publication Date
June 21, 2019 (modified May 16, 2023)
Abstract
Not provided
Cortical neurons projecting to the pontine nuclei. The image was acquired by an all-in-one fluorescent microscope (BZ-X800, Keyence). Green: native GCaMP6s fluorescence. Retrogradely labelled pontine-projecting cortical neurons.
Caption: Cortical neurons projecting to the pontine nuclei. The image was acquired by an all-in-one fluorescent microscope (BZ-X800, Keyence). Green: native GCaMP6s fluorescence. Retrogradely labelled pontine-projecting cortical neurons.
Image attribution: Retrogradely labelled pontine-projecting cortical neurons.
Pontine nucleus in brainstem of mouse. The image was acquired by All-in-one fluorescent microscope (BZ-X800, Keyence). Green: GCaMP6s axons from cortex, blue: native EBFP, red: fluorescent Nissle (NeuroTrace).
Caption: Pontine nucleus in brainstem of mouse. The image was acquired by All-in-one fluorescent microscope (BZ-X800, Keyence). Green: GCaMP6s axons from cortex, blue: native EBFP, red: fluorescent Nissle (NeuroTrace).
Image attribution: null

Any further use of this image requires permission from the lab who submitted the data.

Vectors Used
AAV pmSyn1-EBFP-Cre
Addgene #51507-AAVrg
AAV-hSyn-FLEX-GCaMP6s

Virus & Injection

Virus 1

Virus Name
AAV pmSyn1-EBFP-Cre
Serotype
AAV Retrograde
Source
Addgene
Catalog Number
51507-AAVrg
Promoter
Synapsin
Cargo Type
Recombinase
Injection Titer / Dose
6.90E+12 GC/mL
Injection Volume
300 nL
Injection Rate
30 nL/min
Injector Material
Not provided
Injection Site / Route
Pontine nuclei, NeuroNames: 617
Additional Details
To label projection neurons to the target region. Injection site (FLEX virus) specific.
View in Addgene Catalog

Virus 2

Virus Name
AAV-hSyn-FLEX-GCaMP6s
Serotype
AAV1
Source
Unknown
Catalog Number
Promoter
Synapsin
Cargo Type
Biosensor
Injection Titer / Dose
1.00E+13 GC/mL
Injection Volume
200 nL
Injection Rate
20 nL/min
Injector Material
Not provided
Injection Site / Route
Cerebral cortex, NeuroNames ID: 39
Additional Details
To label projection neurons. Simultaneously injected.

Other Details

Species
Mouse
Strain or Cell Line
C57/BL6J
Source
Not provided
Age at Injection
8-12 weeks
Time After Injection
2 weeks
Detection Method
Direct fluorescence
Assay & Results
Not Provided
Other Information
The tropism of AAV serotypes, especially about AAVrg.
Tips & Comments
If you prevent the back-flow of viral vector, I recommend the injection speed is set as slower as possible. In our lab, serotype 1 and rg (for antero- and retrograde labelling) generally were used. But I wonder rg virus is strongest expression-level at injection site (that is, anterograde-labelling manner), so I worry about the cytotoxicity. I think the lower titer (~1E11 gc/mL) might be suitable.