pAAV-hSyn-DIO-mCherry in Mouse (AAV8)
Addgene #50459-AAV8
- Data Submitted By
- Qijun Tang and Ali Guler
- Submission Date
- July 25, 2019
- Publication Date
- July 25, 2019 (modified May 16, 2023)
- Abstract
- Not Provided
Any further use of this image requires permission from the lab who submitted the data.
- Vector Used
-
pAAV-hSyn-DIO-mCherry
Addgene #50459-AAV8
Virus & Injection
Virus
- Virus Name
- pAAV-hSyn-DIO-mCherry
- Serotype
- AAV8
- Source
- Addgene
- Catalog Number
- 50459-AAV8
- Promoter
- Synapsin
- Cargo Type
- Control
- Injection Titer / Dose
- 4.00E+12 GC/ml
- Injection Volume
- 500 nl
- Injection Rate
- 100 nl/min
- Injector Material
- Not Provided
- Injection Site / Route
- Suprachiasmatic nucleus NeuroNames ID : 384
- Additional Details
- The suprachiasmatic nucleus (SCN) is targeted; Forebrain: Hypothalamus; This virus is hard to get into SCN. You can see it in the attached image. AAV1 might work better than this (AAV8)
Other Details
- Species
- Mouse
- Strain or Cell Line
- C57/BL6J with Drd1-Cre
- Source
- Not provided
- Other Information
- Cited in: Deletion of GAD67 in dopamine receptor-1 expressing cells causes specific motor deficits (https://doi.org/10.1002/dvg.20405)
- Age at Injection
- 10 weeks
- Time After Injection
- 2 weeks
- Detection Method
- Direct fluorescence
- Assay & Results
- This virus is hard to get into SCN. You can see it in the attached image. AAV1 might work better than this (AAV8).
- Functional Testing
- Not determined, it is for control group
- Tips & Comments
- I will use AAV1