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pAAV-hSyn-DIO-mCherry in Mouse (AAV8)

Addgene #50459-AAV8

Data Submitted By
Ali Guler and Qijun Tang
Submission Date
July 25, 2019
Publication Date
July 25, 2019 (modified May 16, 2023)
Abstract
Not Provided
Caption: blue: DAPI. red: mCherry (no staining). 10x objective

Any further use of this image requires permission from the lab who submitted the data. This scientist responded to a specific request for user data and was offered compensation for time and input.

Vector Used
pAAV-hSyn-DIO-mCherry
#50459-AAV8

Virus & Injection

Virus

Virus Name
pAAV-hSyn-DIO-mCherry
Serotype
AAV8
Catalog Number
50459-AAV8
Promoter
Synapsin
Cargo Type
Control
Injection Titer / Dose
4.00E+12 GC/ml
Injection Volume
500 nl
Injection Rate
100 nl/min
Injector Material
Not Provided
Injection Site / Route
Suprachiasmatic nucleus NeuroNames ID : 384
Additional Details
The suprachiasmatic nucleus (SCN) is targeted; Forebrain: Hypothalamus; This virus is hard to get into SCN. You can see it in the attached image. AAV1 might work better than this (AAV8)
View in Addgene Catalog

Other Details

Species
Mouse
Strain or Cell Line
C57/BL6J with Drd1-Cre
Source
Not provided
Other Information
Cited in: Deletion of GAD67 in dopamine receptor-1 expressing cells causes specific motor deficits (https://doi.org/10.1002/dvg.20405)
Age at Injection
10 weeks
Time After Injection
2 weeks
Detection Method
Direct fluorescence
Assay & Results
This virus is hard to get into SCN. You can see it in the attached image. AAV1 might work better than this (AAV8).
Functional Testing
Not determined, it is for control group
Tips & Comments
I will use AAV1