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pAAV-hSyn-DIO-mCherry in Rat (AAV8)

Addgene #50459-AAV8

Data Submitted By
Todd Nentwig and L. Judson Chandler
Submission Date
August 02, 2019
Publication Date
August 02, 2019 (modified May 16, 2023)
Abstract
Not Provided
Caption: Insular cortex neurons projecting to thalamic/habenular areas labeled using hSyn-DIO-mCherry and retrograde Cre viruses. Endogenous mCherry expression is depicted in red. Immuno-amplified mCherry (Chicken anti-mCherry, AlexaFluor488) expression in green shows finer morphology than endogenous expression.

Any further use of this image requires permission from the lab who submitted the data.

Vectors Used
pAAV-hSyn-DIO-mCherry
Addgene #50459-AAV8
pENN.AAV.hSyn.Cre.WPRE.hGH
Addgene #105553-AAVrg

Virus & Injection

Virus 1

Virus Name
pAAV-hSyn-DIO-mCherry
Serotype
AAV8
Source
Addgene
Catalog Number
50459-AAV8
Promoter
Synapsin
Cargo Type
Control
Injection Titer / Dose
1.00E+13 GC/ml
Injection Volume
500 nL
Injection Rate
5 nL/sec
Injector Material
Not Provided
Injection Site / Route
Insular Cortex, NeuroNames: 3613
Additional Details
Insular Cortex (AP: 3. 0, ML: 3. 8, DV: 6. 0) projection neurons. Insular cortex neurons projecting to thalamic/habenula area.
View in Addgene Catalog

Virus 2

Virus Name
pENN.AAV.hSyn.Cre.WPRE.hGH
Serotype
AAV Retrograde
Source
Addgene
Catalog Number
105553-AAVrg
Promoter
Synapsin
Cargo Type
Recombinase
Injection Titer / Dose
1.20E+13 GC/ml
Injection Volume
200 nl
Injection Rate
60 nl/min
Injector Material
Not Provided
Injection Site / Route
Habenula, NeuroNames ID: 294
View in Addgene Catalog

Other Details

Species
Rat
Strain or Cell Line
Long-Evans
Source
Not provided
Age at Injection
~3 months old
Time After Injection
8 weeks
Detection Method
Immunohistochemistry
Assay & Results
Insular cortex neurons projecting to thalamic/habenular areas labeled using hSyn-DIO-mCherry and retrograde Cre viruses.
Target Cell Transduction
Yes, retrograde Cre mediated expression of the mCherry reporter construct was present in the injected region.