pAAV-hSyn-DIO-mCherry in Rat (AAV8)
Addgene #50459-AAV8
- Data Submitted By
- Todd Nentwig and L. Judson Chandler
- Submission Date
- August 02, 2019
- Publication Date
- August 02, 2019 (modified May 16, 2023)
- Abstract
- Not Provided
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- Vectors Used
-
pAAV-hSyn-DIO-mCherry
Addgene #50459-AAV8 -
pENN.AAV.hSyn.Cre.WPRE.hGH
Addgene #105553-AAVrg
Virus & Injection
Virus 1
- Virus Name
- pAAV-hSyn-DIO-mCherry
- Serotype
- AAV8
- Source
- Addgene
- Catalog Number
- 50459-AAV8
- Promoter
- Synapsin
- Cargo Type
- Control
- Injection Titer / Dose
- 1.00E+13 GC/ml
- Injection Volume
- 500 nL
- Injection Rate
- 5 nL/sec
- Injector Material
- Not Provided
- Injection Site / Route
- Insular Cortex, NeuroNames: 3613
- Additional Details
- Insular Cortex (AP: 3. 0, ML: 3. 8, DV: 6. 0) projection neurons. Insular cortex neurons projecting to thalamic/habenula area.
Virus 2
- Virus Name
- pENN.AAV.hSyn.Cre.WPRE.hGH
- Serotype
- AAV Retrograde
- Source
- Addgene
- Catalog Number
- 105553-AAVrg
- Promoter
- Synapsin
- Cargo Type
- Recombinase
- Injection Titer / Dose
- 1.20E+13 GC/ml
- Injection Volume
- 200 nl
- Injection Rate
- 60 nl/min
- Injector Material
- Not Provided
- Injection Site / Route
- Habenula, NeuroNames ID: 294
Other Details
- Species
- Rat
- Strain or Cell Line
- Long-Evans
- Source
- Not provided
- Age at Injection
- ~3 months old
- Time After Injection
- 8 weeks
- Detection Method
- Immunohistochemistry
- Assay & Results
- Insular cortex neurons projecting to thalamic/habenular areas labeled using hSyn-DIO-mCherry and retrograde Cre viruses.
- Target Cell Transduction
- Yes, retrograde Cre mediated expression of the mCherry reporter construct was present in the injected region.