AAV pCAG-FLEX-EGFP-WPRE in Mouse (AAV1)
Addgene #51502-AAV1
- Data Submitted By
- Kyle Jenks and Jason Shepherd
- Submission Date
- January 02, 2020
- Publication Date
- January 02, 2020 (modified May 16, 2023)
- Abstract
- Not provided
Any further use of this image requires permission from the lab who submitted the data.
- Vectors Used
-
AAV pCAG-FLEX-EGFP-WPRE
Addgene #51502-AAV1 -
pENN.AAV.CamKII 0.4.Cre.SV40
Addgene #105558-AAV1
Virus & Injection
Virus 1
- Virus Name
- AAV pCAG-FLEX-EGFP-WPRE
- Serotype
- AAV1
- Source
- Addgene
- Catalog Number
- 51502-AAV1
- Promoter
- CAG
- Cargo Type
- Control
- Injection Titer / Dose
- 1.00E+13 GC/mL
- Injection Volume
- two 100 nL injections (200 nL total)
- Injection Rate
- 36.8 nL/min
- Injector Material
- Not provided
- Injection Site / Route
- Visual cortex, NeuroNames ID: 2102
- Additional Details
- Binocular Visual Cortex.
Virus 2
- Virus Name
- pENN.AAV.CamKII 0.4.Cre.SV40
- Serotype
- AAV1
- Source
- Addgene
- Catalog Number
- 105558-AAV1
- Promoter
- CaMKII
- Injection Titer / Dose
- 1.00E+13 GC/mL
- Injection Volume
- 100 nL
- Injection Rate
- 36.8 nL/min
- Injector Material
- Not provided
- Injection Site / Route
- Cerebral cortex, NeuroNames ID: 39
- Additional Details
- Excitatory cell types, same surgery; Forebrain: Cerebral cortex (neocortex)
Other Details
- Species
- Mouse
- Strain or Cell Line
- C57BL/6J
- Source
- Not provided
- Age at Injection
- P49
- Time After Injection
- 2 weeks
- Detection Method
- Direct fluorescence
- Assay & Results
- Spiny projection neurons successfully labeled. Poor visibility in immediate injection site when using in vivo two-photon imaging. Binocular Visual Cortex, did not confirm cell identity by secondary staining.
- Tips & Comments
- Less/diluted Cre could have been used.