pAAV-CaMKIIa-hM4D(Gi)-mCherry in Mouse (AAV5)
Addgene #50477-AAV5
- Data Submitted By
- Kyle Puhger and Brian Wiltgen
- Submission Date
- June 20, 2019
- Publication Date
- June 20, 2019 (modified May 16, 2023)
- Abstract
- Not Provided
Any further use of this image requires permission from the lab who submitted the data.
- Vector Used
-
pAAV-CaMKIIa-hM4D(Gi)-mCherry
Addgene #50477-AAV5
Virus & Injection
Virus
- Virus Name
- pAAV-CaMKIIa-hM4D(Gi)-mCherry
- Serotype
- AAV5
- Source
- Addgene
- Catalog Number
- 50477-AAV5
- Promoter
- CaMKII
- Cargo Type
- Chemogenetics
- Injection Titer / Dose
- 9.50E+12 GC/ml
- Injection Volume
- 80 nl
- Injection Rate
- 80 nl/min
- Injector Material
- Not Provided
- Injection Site / Route
- Reuniens nucleus, NeuroNames ID: 309
- Additional Details
- Virus was targeted to the thalamic nucleus reuniens.
Other Details
- Species
- Mouse
- Strain or Cell Line
- C57/BL6J
- Source
- Jackson Labs
- Stock Number
- JAX: 000664
- Age at Injection
- 9-12 weeks
- Time After Injection
- 3 weeks
- Detection Method
- Immunohistochemistry
- Assay & Results
- Putative CaMKII+ cells in nucleus reuniens (RE) were labeled.
- Functional Testing
- CNO was used to activate the receptor.
- Tips & Comments
- Virus expression was very strong, I would have liked to perform a range of dilutions.