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pAAV-CaMKIIa-hM4D(Gi)-mCherry in Mouse (AAV5)

Addgene #50477-AAV5

Data Submitted By
Kyle Puhger and Brian Wiltgen
Submission Date
June 20, 2019
Publication Date
June 20, 2019 (modified May 16, 2023)
Abstract
Not Provided
Caption: Images were acquired at 20× magnification using a fluorescence slide scanner (BX61VS, Olympus).

Any further use of this image requires permission from the lab who submitted the data.

Vector Used
pAAV-CaMKIIa-hM4D(Gi)-mCherry
Addgene #50477-AAV5

Virus & Injection

Virus

Virus Name
pAAV-CaMKIIa-hM4D(Gi)-mCherry
Serotype
AAV5
Source
Addgene
Catalog Number
50477-AAV5
Promoter
CaMKII
Cargo Type
Chemogenetics
Injection Titer / Dose
9.50E+12 GC/ml
Injection Volume
80 nl
Injection Rate
80 nl/min
Injector Material
Not Provided
Injection Site / Route
Reuniens nucleus, NeuroNames ID: 309
Additional Details
Virus was targeted to the thalamic nucleus reuniens.
View in Addgene Catalog

Other Details

Species
Mouse
Strain or Cell Line
C57/BL6J
Source
Jackson Labs
Stock Number
JAX: 000664
Age at Injection
9-12 weeks
Time After Injection
3 weeks
Detection Method
Immunohistochemistry
Assay & Results
Putative CaMKII+ cells in nucleus reuniens (RE) were labeled.
Functional Testing
CNO was used to activate the receptor.
Tips & Comments
Virus expression was very strong, I would have liked to perform a range of dilutions.