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pAAV-hSyn-DIO-hM3D(Gq)-mCherry in Mouse (AAV2)

Addgene #44361-AAV2

Data Submitted By
Yanghui Sheng and King-Wai Yau
Submission Date
June 19, 2019
Publication Date
June 19, 2019 (modified May 16, 2023)
Abstract
Not Provided
Caption: Green: immuno-staining with an antibody against melanopsin; Red: fluorescence signal from hM3D-mCherry.

Any further use of this image requires permission from the lab who submitted the data.

Vector Used
pAAV-hSyn-DIO-hM3D(Gq)-mCherry
Addgene #44361-AAV2

Virus & Injection

Virus

Virus Name
pAAV-hSyn-DIO-hM3D(Gq)-mCherry
Serotype
AAV2
Source
Addgene
Catalog Number
44361-AAV2
Promoter
Synapsin
Cargo Type
Chemogenetics
Injection Titer / Dose
5.00E+12 GC/ml
Injection Volume
2 μl per eye
Injection Rate
Not Provided
Injector Material
Not Provided
Injection Site / Route
Retina, NeuroNames ID : 1862
Additional Details
Targeting retinal ganglion cells. Virus was injected into Opn4-Cre mice. Melanopsin-expressing intrinsically photosensitive retinal ganglion cells ended up expressing hM3D from viral infection
View in Addgene Catalog

Other Details

Species
Mouse
Strain or Cell Line
C57/BL6J background (Opn4-Cre is a BAC-transgene that we inserted into the mouse genome so that Cre recombinase is expressed in Opn4-positive cells)
Source
Not provided
Other Information
Virus was injected into Opn4-Cre mice.
Age at Injection
1-3 months
Time After Injection
8 weeks
Detection Method
Direct fluorescence
Assay & Results
Melanopsin-expressing intrinsically photosensitive retinal ganglion cells ended up expressing hM3D from viral infection.