pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40 in Rat (AAV1)

Addgene #98928-AAV1

Data Submitted By: L. Judson Chandler and Todd Nentwig
Submission Date: July 26, 2019
Publication Date: July 26, 2019 (modified May 16, 2023)
Abstract: Not Provided

**Caption:** Apical dendrite of Prelimbic cortex projection neurons expressing AAV1.FLeX.sm-Flag. Immunolabeling was used to identify the following targets -- Flag: Red; cFos: Green; DAPI: Blue. A Zeiss 880 confocal microscope (20x mag,) was used to obtain this image.

Any further use of this image requires permission from the lab who submitted the data. This scientist responded to a specific request for user data and was offered compensation for time and input.

Vectors Used: pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40
#98928-AAV1; pENN.AAV.hSyn.Cre.WPRE.hGH
#105553-AAVrg

Virus & Injection

Virus 1

Virus Name: pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40
Serotype: AAV1
Catalog Number: 98928-AAV1
Promoter: CAG
Cargo Type: Control
Injection Titer / Dose: 2.20E+13 GC/mL
Injection Volume: 500 nL each in prelimibic and infralimbic cortices
Injection Rate: 250 nL/min
Injector Material: Not Provided
Injection Site / Route: Prelimbic area, NeuroNames ID: 1078
Additional Details: Injections were targeted at the prelimbic and Infralimbic cortex projection neurons.

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Virus 2

Virus Name: pENN.AAV.hSyn.Cre.WPRE.hGH
Serotype: AAV Retrograde
Catalog Number: 105553-AAVrg
Promoter: Synapsin
Injection Titer / Dose: 1.20E+13 GC/mL
Injection Volume: 200 nL
Injection Rate: 60 nL/min
Injector Material: Not Provided
Injection Site / Route: Epithalamus, NeuroNames ID: 292
Additional Details: Targeting neurons using a pan-neuronal hSyn promoter.

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Other Details

Species: Rat
Strain or Cell Line: Long-Evans
Source: Not provided
Age at Injection: ~100 days old
Time After Injection: ~10 weeks
Detection Method: Immunohistochemistry
Assay & Results: Prelimbic and Infralimibic projection neurons were targeted and labeled with the protein encoded by the virus used.
Target Cell Transduction: Yes, the cells were labeled well with the Cre-dependent reporter virus.
Other Information: No toxicity or inflammation were observed.
Tips & Comments: At the present time, I have no additional comments. The virus works as described and expected based on the initial report (Viswanathan et al, 2015).