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pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40 in Rat (AAV1)

Addgene #98928-AAV1

Data Submitted By
L. Judson Chandler and Todd Nentwig
Submission Date
July 26, 2019
Publication Date
July 26, 2019 (modified May 16, 2023)
Abstract
Not Provided
Caption: Apical dendrite of Prelimbic cortex projection neurons expressing AAV1.FLeX.sm-Flag. Immunolabeling was used to identify the following targets -- Flag: Red; cFos: Green; DAPI: Blue. A Zeiss 880 confocal microscope (20x mag,) was used to obtain this image.

Any further use of this image requires permission from the lab who submitted the data. This scientist responded to a specific request for user data and was offered compensation for time and input.

Vectors Used
pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40
#98928-AAV1
pENN.AAV.hSyn.Cre.WPRE.hGH
#105553-AAVrg

Virus & Injection

Virus 1

Virus Name
pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40
Serotype
AAV1
Catalog Number
98928-AAV1
Promoter
CAG
Cargo Type
Control
Injection Titer / Dose
2.20E+13 GC/mL
Injection Volume
500 nL each in prelimibic and infralimbic cortices
Injection Rate
250 nL/min
Injector Material
Not Provided
Injection Site / Route
Prelimbic area, NeuroNames ID: 1078
Additional Details
Injections were targeted at the prelimbic and Infralimbic cortex projection neurons.
View in Addgene Catalog

Virus 2

Virus Name
pENN.AAV.hSyn.Cre.WPRE.hGH
Serotype
AAV Retrograde
Catalog Number
105553-AAVrg
Promoter
Synapsin
Injection Titer / Dose
1.20E+13 GC/mL
Injection Volume
200 nL
Injection Rate
60 nL/min
Injector Material
Not Provided
Injection Site / Route
Epithalamus, NeuroNames ID: 292
Additional Details
Targeting neurons using a pan-neuronal hSyn promoter.
View in Addgene Catalog

Other Details

Species
Rat
Strain or Cell Line
Long-Evans
Source
Not provided
Age at Injection
~100 days old
Time After Injection
~10 weeks
Detection Method
Immunohistochemistry
Assay & Results
Prelimbic and Infralimibic projection neurons were targeted and labeled with the protein encoded by the virus used.
Target Cell Transduction
Yes, the cells were labeled well with the Cre-dependent reporter virus.
Other Information
No toxicity or inflammation were observed.
Tips & Comments
At the present time, I have no additional comments. The virus works as described and expected based on the initial report (Viswanathan et al, 2015).