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pENN.AAV.CamKII.GCaMP6f.WPRE.SV40 in Mouse (AAV9)

Addgene #100834-AAV9

Data Submitted By
Brian Wiltgen and Kyle Puhger
Submission Date
June 20, 2019
Publication Date
June 20, 2019 (modified May 16, 2023)
Abstract
Not Provided
Caption: Images were acquired at 20× magnification using a fluorescence slide scanner (BX61VS, Olympus).
Image attribution: Kyle Puhger

Any further use of this image requires permission from the lab who submitted the data. This scientist responded to a specific request for user data and was offered compensation for time and input.

Vector Used
pENN.AAV.CamKII.GCaMP6f.WPRE.SV40
#100834-AAV9

Virus & Injection

Virus

Virus Name
pENN.AAV.CamKII.GCaMP6f.WPRE.SV40
Serotype
AAV9
Catalog Number
100834-AAV9
Promoter
CaMKII
Cargo Type
Biosensor
Injection Titer / Dose
2.50E+13 GC/mL
Injection Volume
250 nL
Injection Rate
160 nL/min
Injector Material
Not Provided
Injection Site / Route
CA1 field, NeuroNames ID: 185
View in Addgene Catalog

Other Details

Species
Mouse
Strain or Cell Line
C57/BL6J
Source
Jackson Labs
Stock Number
JAX: 000664
Age at Injection
9-12 weeks
Time After Injection
4 weeks
Detection Method
Immunohistochemistry, Direct fluorescence
Assay & Results
Putative CaMKII+ pyramidal cells in CA1 subregion.
Functional Testing
GCaMP signals in dorsal CA1 were collected via fiber photometry.