Return to AAV Results

pENN.AAV.CamKII.GCaMP6f.WPRE.SV40 in Mouse (AAV9)

Addgene #100834-AAV9

Data Submitted By: Kyle Puhger and Brian Wiltgen
Submission Date: June 20, 2019
Publication Date: June 20, 2019 (modified May 16, 2023)
Abstract: Not Provided

Images were acquired at 20× magnification using a fluorescence slide scanner (BX61VS, Olympus). — Caption: Images were acquired at 20× magnification using a fluorescence slide scanner (BX61VS, Olympus).
Image attribution: Kyle Puhger

Any further use of this image requires permission from the lab who submitted the data.

Vector Used: pENN.AAV.CamKII.GCaMP6f.WPRE.SV40
Addgene #100834-AAV9

Virus & Injection

Virus

Virus Name: pENN.AAV.CamKII.GCaMP6f.WPRE.SV40
Serotype: AAV9
Source: Addgene
Catalog Number: 100834-AAV9
Promoter: CaMKII
Cargo Type: Biosensor
Injection Titer / Dose: 2.50E+13 GC/mL
Injection Volume: 250 nL
Injection Rate: 160 nL/min
Injector Material: Not Provided
Injection Site / Route: CA1 field, NeuroNames ID: 185

View in Addgene Catalog

Other Details

Species: Mouse
Strain or Cell Line: C57/BL6J
Source: Jackson Labs
Stock Number: JAX: 000664
Age at Injection: 9-12 weeks
Time After Injection: 4 weeks
Detection Method: Immunohistochemistry, Direct fluorescence
Assay & Results: Putative CaMKII+ pyramidal cells in CA1 subregion.
Functional Testing: GCaMP signals in dorsal CA1 were collected via fiber photometry.