pAAV-hSyn-DIO-hM4D(Gi)-mCherry in Rat (AAV8)
Addgene #44362-AAV8
- Data Submitted By
- Elizabeth Crummy and Susan Ferguson
- Submission Date
- June 20, 2019
- Publication Date
- June 20, 2019 (modified May 16, 2023)
- Abstract
- Not Provided
Any further use of this image requires permission from the lab who submitted the data.
- Vectors Used
-
pAAV-hSyn-DIO-hM4D(Gi)-mCherry
Addgene #44362-AAV8 - CAV2-CRE
Virus & Injection
Virus 1
- Virus Name
- pAAV-hSyn-DIO-hM4D(Gi)-mCherry
- Serotype
- AAV8
- Source
- Addgene
- Catalog Number
- 44362-AAV8
- Promoter
- Synapsin
- Cargo Type
- Chemogenetics
- Injection Titer / Dose
- 2.00E+13 GC/mL
- Injection Volume
- 750 nL
- Injection Rate
- 150 nL/min
- Injector Material
- Not Provided
- Injection Site / Route
- Orbital gyri, NeuroNames ID: 91
- Additional Details
- Pyramidal tract neurons in the orbitofrontal cortex (CRE injected in the pons).
Virus 2
- Virus Name
- CAV2-CRE
- Serotype
- N/A
- Source
- Unknown
- Catalog Number
- N/A
- Promoter
- Other
- Other Promoter
- Not Provided.
- Injection Titer / Dose
- Not Provided
- Injection Volume
- 1 μL injections
- Injection Rate
- 200 nL/min
- Injector Material
- Not Provided
- Injection Site / Route
- Pons, NeuroNames ID: 547
- Additional Details
- Pyramidal tract neurons. Surgeries for both CAV and AAV injections were performed the same day. The order of injections was alternated.
Other Details
- Species
- Rat
- Strain or Cell Line
- Sprague Dawley; Long Evans
- Source
- Not provided
- Age at Injection
- ~2-3 months
- Time After Injection
- 2 weeks
- Detection Method
- Immunohistochemistry
- Assay & Results
- Around lateral and ventral orbital cortex.
- Functional Testing
- CNO (5 mg/kg) was used for transient inhibition of pyramidal tract neurons. Within subject controls were utilized. Expression was successfully verified with living colors dsRed rabbit polyclonal antibody.