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pAAV.hSyn.Flex.iGluSnFr.WPRE.SV40 in Mouse (AAV1)

Addgene #98931-AAV1

Data Submitted By
Sam Benezra and Randy Bruno
Submission Date
July 09, 2019
Publication Date
July 09, 2019 (modified May 16, 2023)
Abstract
Not Provided
Caption: Green channel, Sutter MOM, Zeiss 20x 1.0NA objective

Any further use of this image requires permission from the lab who submitted the data.

Vector Used
pAAV.hSyn.Flex.iGluSnFr.WPRE.SV40
Addgene #98931-AAV1

Virus & Injection

Virus

Virus Name
pAAV.hSyn.Flex.iGluSnFr.WPRE.SV40
Serotype
AAV1
Source
Addgene
Catalog Number
98931-AAV1
Promoter
Synapsin
Cargo Type
Biosensor
Injection Titer / Dose
7.67E+12 GC/ml
Injection Volume
2 injection sites, 200 nl each site
Injection Rate
30 nl/min
Injector Material
Not Provided
Injection Site / Route
Whisker sensory area (Barrel Cortex), NeuroNames ID: 2703
Additional Details
Layer 5 neocortex; Forebrain: Cerebral cortex (neocortex); Layer 5 pyramidal neurons in S1 barrel field. This was an RPP4-cre mouse, which sparsely labels layer 5 pyramidal cells
View in Addgene Catalog

Other Details

Species
Mouse
Strain or Cell Line
C57/BL6J
Source
Not provided
Age at Injection
10 weeks
Time After Injection
3 weeks
Detection Method
Direct fluorescence
Assay & Results
No toxicity or inflammation. The prep lasted over 3 months.
Functional Testing
Air puff stimuli were directed at the whisker, which evoked GluSnFR activity in layer 5 apical dendrites.
Other Information
No toxicity or inflammation. The prep lasted over 3 months
Tips & Comments
No, this virus worked exactly as expected