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pAAV-hSyn-dF-HA-KORD-IRES-mCitrine in Rat (AAV8)

Addgene #65417-AAV8

Data Submitted By
Christina Ruiz and Stephen V Mahler
Submission Date
June 25, 2019
Publication Date
June 25, 2019 (modified May 16, 2023)
Abstract
Not Provided
Caption: anti-mCherry (DIO-hM4D(Gi) tag) in red, anti-GFP (dF-KORD tag) in green, and anti-TH in blue brightfield/fluorescent microscope, 20x objective
Image attribution: Erik Castillo

Any further use of this image requires permission from the lab who submitted the data.

Vectors Used
pAAV-hSyn-dF-HA-KORD-IRES-mCitrine
Addgene #65417-AAV8
pAAV-hSyn-DIO-hM4D(Gi)-mCherry
Addgene #44362-AAV2

Virus & Injection

Virus 1

Virus Name
pAAV-hSyn-dF-HA-KORD-IRES-mCitrine
Serotype
AAV8
Source
Addgene
Catalog Number
65417-AAV8
Promoter
Synapsin
Cargo Type
Chemogenetics
Injection Titer / Dose
7.00E+12 GC/mL
Injection Volume
600 nL
Injection Rate
600 nL/min
Injector Material
Not Provided
Injection Time
5 min
Injection Site / Route
Ventral tegmental area, NeuroNames ID: 521
Additional Details
Since injection was at the same site, both viruses were mixed 1:1 ratio and then a total of 1.2 µl was injected to give 600 nl of each virus. We waited 5 min before removing the injection pipette from the injection site.
View in Addgene Catalog

Virus 2

Virus Name
pAAV-hSyn-DIO-hM4D(Gi)-mCherry
Serotype
AAV2
Source
Addgene
Catalog Number
44362-AAV2
Promoter
Synapsin
Cargo Type
Chemogenetics
Injection Titer / Dose
5.00E+12 GC/mL
Injection Volume
600 nL
Injection Rate
600 nL/min
Injector Material
Not Provided
Injection Site / Route
Ventral tegmental area, NeuroNames ID: 521
Additional Details
Targeting dopamine neurons in Ventral Tegmental Area.
View in Addgene Catalog

Other Details

Species
Rat
Strain or Cell Line
Long Evans THcre
Source
Rat Resource & Research Center
Stock Number
659
Other Information
Long Evans-Tg(TH-Cre)3.1Deis
Age at Injection
postnatal day 60-90
Time After Injection
3 weeks
Detection Method
Immunofluorescence for mCherry, GFP, and TH
Assay & Results
Not Provided
Other Information
No toxicity or inflammation seen.