pAAV-hSyn-dF-HA-KORD-IRES-mCitrine in Rat (AAV8)
Addgene #65417-AAV8
- Data Submitted By
- Christina Ruiz and Stephen V Mahler
- Submission Date
- June 25, 2019
- Publication Date
- June 25, 2019 (modified May 16, 2023)
- Abstract
- Not Provided
Image attribution: Erik Castillo
Any further use of this image requires permission from the lab who submitted the data.
- Vectors Used
-
pAAV-hSyn-dF-HA-KORD-IRES-mCitrine
Addgene #65417-AAV8 -
pAAV-hSyn-DIO-hM4D(Gi)-mCherry
Addgene #44362-AAV2
Virus & Injection
Virus 1
- Virus Name
- pAAV-hSyn-dF-HA-KORD-IRES-mCitrine
- Serotype
- AAV8
- Source
- Addgene
- Catalog Number
- 65417-AAV8
- Promoter
- Synapsin
- Cargo Type
- Chemogenetics
- Injection Titer / Dose
- 7.00E+12 GC/mL
- Injection Volume
- 600 nL
- Injection Rate
- 600 nL/min
- Injector Material
- Not Provided
- Injection Time
- 5 min
- Injection Site / Route
- Ventral tegmental area, NeuroNames ID: 521
- Additional Details
- Since injection was at the same site, both viruses were mixed 1:1 ratio and then a total of 1.2 µl was injected to give 600 nl of each virus. We waited 5 min before removing the injection pipette from the injection site.
Virus 2
- Virus Name
- pAAV-hSyn-DIO-hM4D(Gi)-mCherry
- Serotype
- AAV2
- Source
- Addgene
- Catalog Number
- 44362-AAV2
- Promoter
- Synapsin
- Cargo Type
- Chemogenetics
- Injection Titer / Dose
- 5.00E+12 GC/mL
- Injection Volume
- 600 nL
- Injection Rate
- 600 nL/min
- Injector Material
- Not Provided
- Injection Site / Route
- Ventral tegmental area, NeuroNames ID: 521
- Additional Details
- Targeting dopamine neurons in Ventral Tegmental Area.
Other Details
- Species
- Rat
- Strain or Cell Line
- Long Evans THcre
- Source
- Rat Resource & Research Center
- Stock Number
- 659
- Other Information
- Long Evans-Tg(TH-Cre)3.1Deis
- Age at Injection
- postnatal day 60-90
- Time After Injection
- 3 weeks
- Detection Method
- Immunofluorescence for mCherry, GFP, and TH
- Assay & Results
- Not Provided
- Other Information
- No toxicity or inflammation seen.