pAAV-hSyn-hM4D(Gi)-mCherry in Rat (AAV8)
Addgene #50475-AAV8
- Data Submitted By
- Michael Elbaz and Martin Deschenes
- Submission Date
- August 22, 2019
- Publication Date
- August 22, 2019 (modified May 16, 2023)
- Abstract
- Not Provided
Any further use of this image requires permission from the lab who submitted the data.
- Vector Used
-
pAAV-hSyn-hM4D(Gi)-mCherry
Addgene #50475-AAV8
Virus & Injection
Virus
- Virus Name
- pAAV-hSyn-hM4D(Gi)-mCherry
- Serotype
- AAV8
- Source
- Addgene
- Catalog Number
- 50475-AAV8
- Promoter
- Synapsin
- Cargo Type
- Chemogenetics
- Injection Titer / Dose
- 4.26E+12 GC/mL
- Injection Volume
- 400 nL
- Injection Rate
- Not Provided
- Injector Material
- Not Provided
- Injection Site / Route
- Ventral tegmental area, NeuroNames ID: 521
Other Details
- Species
- Rat
- Strain or Cell Line
- Wild-type Long Evans
- Source
- Not provided
- Age at Injection
- 10 weeks
- Time After Injection
- Not Provided
- Detection Method
- Direct fluorescence
- Assay & Results
- Ventral tegmental area was targeted. Ubiquitous, as expected.
- Functional Testing
- Once injected, rats were trained to a task which, on average, requires a 4-week long training to become proficient. from then on, i.e. in expert rats, we started taking advantage of the DREADDS expression.