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pAAV-CAG-tdTomato (codon diversified) in Mouse (PHP.eB)

Addgene #59462-PHPeB

Data Submitted By
David Brann and Sandeep R Datta
Submission Date
June 26, 2019
Publication Date
June 26, 2019 (modified May 16, 2023)
Abstract
Not Provided
Caption: tdTomato in green; NeuroTrace 640 in magenta. Imaged on Olympus Vs120 slide scanner with 10x objective, 60 ms exposure

Any further use of this image requires permission from the lab who submitted the data. This scientist responded to a specific request for user data and was offered compensation for time and input.

Vector Used
pAAV-CAG-tdTomato (codon diversified)
#59462-PHPeB

Virus & Injection

Virus

Virus Name
pAAV-CAG-tdTomato (codon diversified)
Serotype
PHP.eB
Catalog Number
59462-PHPeB
Promoter
CAG
Cargo Type
Control
Injection Titer / Dose
1.00E+12 GC/ml
Injection Volume
100 μl
Injection Rate
N/A
Injector Material
Via 0. 3 mL insulin syringe
Injection Time
N/A
Injection Site / Route
Intravenous, Retro-orbital vein
Additional Details
Retro-orbital injection to target cells throughout the brain with the PHP. EB serotype.
View in Addgene Catalog

Other Details

Species
Mouse
Strain or Cell Line
C57/BL6J
Source
Jackson Labs
Stock Number
JAX: 000664
Age at Injection
8 weeks
Time After Injection
3 weeks
Detection Method
Direct fluorescence
Assay & Results
Widespread infection throughout the brain. In both excitatory pyramidal neurons, as well as glia. Density was fairly uniform across cortical regions
Other Information
I didn't quantify it, but the percentage of glia infected varied across mice. I didn't see any signs of toxicity (e. G. No signs of blebbing in axons), but I also only had tdTomato
Tips & Comments
PHP. EB + CAG seems like a great combination for transducing a large numbers of neurons across the brain