pAAV-CaMKIIa-hM4D(Gi)-mCherry in Mouse (AAV8)
Addgene #50477-AAV8
- Data Submitted By
- Brittney Cox and Christine Gall
- Submission Date
- June 19, 2019
- Publication Date
- June 19, 2019 (modified May 16, 2023)
- Abstract
- Not Provided
Any further use of this image requires permission from the lab who submitted the data.
- Vector Used
-
pAAV-CaMKIIa-hM4D(Gi)-mCherry
Addgene #50477-AAV8
Virus & Injection
Virus
- Virus Name
- pAAV-CaMKIIa-hM4D(Gi)-mCherry
- Serotype
- AAV8
- Source
- Addgene
- Catalog Number
- 50477-AAV8
- Promoter
- CaMKII
- Cargo Type
- Chemogenetics
- Injection Titer / Dose
- 2.60E+12 GC/ml
- Injection Volume
- 3 μl for hippocampal subregions, 8 μl for entorhial cortex regions
- Injection Rate
- Not Provided
- Injector Material
- Not Provided
- Injection Site / Route
- Hippocampus NeuroNames ID : 3157
- Additional Details
- I specifically injected CA1, CA3, and dentate gyrus. Additionally, lateral and medial entorhinal cortex; Forebrain: Hippocampus; CA1 & CA3=pyramidal cells. Dentate gyrus= granual cells. Entorhinal cortex= pyramidal cells
Other Details
- Species
- Mouse
- Strain or Cell Line
- Wild type FVB129
- Source
- Not provided
- Age at Injection
- 2 months
- Time After Injection
- 3 weeks
- Detection Method
- Immunohistochemistry
- Assay & Results
- Not Provided
- Functional Testing
- CNO was used at 1 mg/kg systemically and 10 uM in slice electrophysiology
- Tips & Comments
- Previously tried serotype 5 for these experiments and we got some retrograde labeling, so 8 worked best