pGP-AAV-syn-jGCaMP7f-WPRE in Mouse (AAV Retrograde)
Addgene #104488-AAVrg
- Data Submitted By
- Hongbin Yang and Stephan Lammel
- Submission Date
- June 18, 2020
- Publication Date
- June 18, 2020 (modified May 16, 2023)
- Abstract
- Not Provided
Any further use of this image requires permission from the lab who submitted the data. This scientist responded to a specific request for user data and was offered compensation for time and input.
- Vector Used
-
pGP-AAV-syn-jGCaMP7f-WPRE
#104488-AAVrg
Virus & Injection
Virus
- Virus Name
- pGP-AAV-syn-jGCaMP7f-WPRE
- Serotype
- AAV Retrograde
- Catalog Number
- 104488-AAVrg
- Promoter
- Synapsin
- Cargo Type
- Biosensor
- Injection Titer / Dose
- 7.00E+12 GC/mL
- Injection Volume
- 400 nL
- Injection Rate
- 100 nL/min
- Injector Material
- Not Provided
- Injection Site / Route
- Pars reticulata, NeuroNames ID: 538
Other Details
- Species
- Mouse
- Strain or Cell Line
- mouse
- Source
- Not provided
- Age at Injection
- 8 weeks
- Time After Injection
- 3 weeks
- Detection Method
- Immunohistochemistry
- Assay & Results
- Lateral SNR including VTA and SNR neurons. Midbrain tegmentum area neurons expressing retro-AAV-jGCaMP7f. Immunostaining was used to identify the following targets—GFP: GcaMP7f (green); TH: Dopamine neurons (blue).
- Functional Testing
- It worked as expected
- Other Information
- This virus has low toxicity and works really well for measuring neuronal activity.