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pGP-AAV-syn-jGCaMP7f-WPRE in Mouse (AAV Retrograde)

Addgene #104488-AAVrg

Data Submitted By
Hongbin Yang and Stephan Lammel
Submission Date
June 18, 2020
Publication Date
June 18, 2020 (modified May 16, 2023)
Abstract
Not Provided
Caption: Midbrain tegmentum area neurons expressing retro-AAV-jGCaMP7f. Immunostaining was used to identify the following targets—GFP: GcaMP7f (green); TH: Dopamine neurons (blue). A Zeiss AxioImager M2 upright widefield fluorescence/differential interference contrast microscope (5x objective) was used to obtain the image.

Any further use of this image requires permission from the lab who submitted the data. This scientist responded to a specific request for user data and was offered compensation for time and input.

Vector Used
pGP-AAV-syn-jGCaMP7f-WPRE
#104488-AAVrg

Virus & Injection

Virus

Virus Name
pGP-AAV-syn-jGCaMP7f-WPRE
Serotype
AAV Retrograde
Catalog Number
104488-AAVrg
Promoter
Synapsin
Cargo Type
Biosensor
Injection Titer / Dose
7.00E+12 GC/mL
Injection Volume
400 nL
Injection Rate
100 nL/min
Injector Material
Not Provided
Injection Site / Route
Pars reticulata, NeuroNames ID: 538
View in Addgene Catalog

Other Details

Species
Mouse
Strain or Cell Line
mouse
Source
Not provided
Age at Injection
8 weeks
Time After Injection
3 weeks
Detection Method
Immunohistochemistry
Assay & Results
Lateral SNR including VTA and SNR neurons. Midbrain tegmentum area neurons expressing retro-AAV-jGCaMP7f. Immunostaining was used to identify the following targets—GFP: GcaMP7f (green); TH: Dopamine neurons (blue).
Functional Testing
It worked as expected
Other Information
This virus has low toxicity and works really well for measuring neuronal activity.