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pAAV-CamKII-ArchT-GFP (PV2527) in Zebra finch (AAV1)

Addgene #99039-AAV1

Data Submitted By
Jonnathan Singh Alvarado and Richard Mooney
Submission Date
August 18, 2019
Publication Date
August 18, 2019 (modified May 16, 2023)
Abstract
Not Provided
488 Green = Anti GFP for labeling ArchT expression. 594 Red = DARPP32 Marker for Spiny Neurons Zeiss 710 Inverted Confocal Microscope 20x Magnification Tear down the middle is optogenetic probe placement after experiment completion.
Caption: 488 Green = Anti GFP for labeling ArchT expression. 594 Red = DARPP32 Marker for Spiny Neurons Zeiss 710 Inverted Confocal Microscope 20x Magnification Tear down the middle is optogenetic probe placement after experiment completion.

Any further use of this image requires permission from the lab who submitted the data.

Vector Used
pAAV-CamKII-ArchT-GFP (PV2527)
Addgene #99039-AAV1

Virus & Injection

Virus

Virus Name
pAAV-CamKII-ArchT-GFP (PV2527)
Serotype
AAV1
Source
Addgene
Catalog Number
99039-AAV1
Promoter
CaMKII
Cargo Type
Optogenetics
Injection Titer / Dose
2.00E+12 GC/ml
Injection Volume
350 nl
Injection Rate
Not Provided
Injector Material
Not Provided
Injection Site / Route
Area X NeuroNames ID : 342
Additional Details
Targetting Medium Spiny Neurons In Area X; Forebrain: Basal Ganglia: Striatum (Putamen or caudate); Area X, HVC
View in Addgene Catalog

Other Details

Species
Other
Other Species
Zebra Finch
Strain or Cell Line
N/A
Source
Not provided
Age at Injection
100 days
Time After Injection
3 weeks
Detection Method
Immunohistochemistry
Assay & Results
Not Provided
Functional Testing
Light-induced suppression detected in anesthetized bird