pAAV-hSyn-DIO-hM3D(Gq)-mCherry in Mouse (AAV8)
Addgene #44361-AAV8
- Data Submitted By
- Qijun Tang and Ali Guler
- Submission Date
- June 19, 2019
- Publication Date
- June 19, 2019 (modified May 16, 2023)
- Abstract
- Not Provided
Any further use of this image requires permission from the lab who submitted the data.
- Vector Used
-
pAAV-hSyn-DIO-hM3D(Gq)-mCherry
Addgene #44361-AAV8
Virus & Injection
Virus
- Virus Name
- pAAV-hSyn-DIO-hM3D(Gq)-mCherry
- Serotype
- AAV8
- Source
- Addgene
- Catalog Number
- 44361-AAV8
- Promoter
- Synapsin
- Cargo Type
- Chemogenetics
- Injection Titer / Dose
- 4.00E+12 GC/ml
- Injection Volume
- 500 nl
- Injection Rate
- 100 nl/min
- Injector Material
- Not Provided
- Injection Site / Route
- Suprachiasmatic nucleus, NeuroNames ID : 384 Paraventricular nucleus of the hypothalamus, NeuroNames ID : 387
- Additional Details
- Targeting Drd1 cells in suprachiamatic nucleus (SCN); Forebrain: Hypothalamus; Targeted region (SCN) and part of PVN
Other Details
- Species
- Mouse
- Strain or Cell Line
- C57/BL6J
- Source
- Not provided
- Age at Injection
- 10 weeks
- Time After Injection
- 2 weeks
- Detection Method
- Immunohistochemistry, Direct fluorescence
- Assay & Results
- Not Provided
- Functional Testing
- Yes. 0. 3 mg/kg CNO ip. Injection produces expected behavior and c-fos expression.
- Tips & Comments
- AAV8 does not penetrate SCN as good as AAV1