pAAV-CaMKIIa-hChR2(H134R)-EYFP in Mouse (AAV9)
Addgene #26969-AAV9
- Data Submitted By
- David Haggerty and Brady Atwood
- Submission Date
- August 05, 2019
- Publication Date
- August 05, 2019 (modified May 16, 2023)
- Abstract
- Not Provided
Image attribution: David L. Haggerty & Brady Atwood
Any further use of this image requires permission from the lab who submitted the data.
- Vector Used
-
pAAV-CaMKIIa-hChR2(H134R)-EYFP
Addgene #26969-AAV9
Virus & Injection
Virus
- Virus Name
- pAAV-CaMKIIa-hChR2(H134R)-EYFP
- Serotype
- AAV9
- Source
- Addgene
- Catalog Number
- 26969-AAV9
- Promoter
- CaMKII
- Cargo Type
- Optogenetics
- Injection Titer / Dose
- 1.00E+13 GC/ml
- Injection Volume
- 50 nl
- Injection Rate
- 25 nl/min
- Injector Material
- Not Provided
- Injection Site / Route
- Insular Cortex, NeuroNames: 3613
- Additional Details
- Anterior Insular Cortex - CamkII positive neurons
Other Details
- Species
- Mouse
- Strain or Cell Line
- C57/BL6J
- Source
- Jackson Labs
- Age at Injection
- 6 weeks
- Time After Injection
- 3 weeks
- Detection Method
- Direct fluorescence
- Assay & Results
- Not Provided
- Functional Testing
- Light was used to activate receptors - wireless optogenetics- Neurolux INC
- Other Information
- No inflammation or cell death
- Tips & Comments
- AAV9 has worked the best in our experience. The virus we use is undiluted, and has great expression. We leave the needle in the brain for 2 min post injection and retract slowly, which helps with limiting viral particles spreading from the injection site