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pAAV-CaMKIIa-hM4D(Gi)-mCherry in Mouse (AAV8)

Addgene #50477-AAV8

Data Submitted By
Robert Stackman and Robert W. Stackman
Submission Date
June 26, 2019
Publication Date
June 26, 2019 (modified May 16, 2023)
Abstract
Not Provided
Caption: Image obtained on standard Nikon Fluorescent microscope, 10x objective, Texas Red channel/filter cube
Image attribution: David Cinalli

Any further use of this image requires permission from the lab who submitted the data. This scientist responded to a specific request for user data and was offered compensation for time and input.

Vector Used
pAAV-CaMKIIa-hM4D(Gi)-mCherry
#50477-AAV8

Virus & Injection

Virus

Virus Name
pAAV-CaMKIIa-hM4D(Gi)-mCherry
Serotype
AAV8
Catalog Number
50477-AAV8
Promoter
CaMKII
Cargo Type
Chemogenetics
Injection Titer / Dose
1.60E+13 GC/mL
Injection Volume
two 0.5 μL injections
Injection Rate
167 nL/min
Injector Material
Not Provided
Injection Site / Route
CA1 field, NeuroNames ID: 185
View in Addgene Catalog

Other Details

Species
Mouse
Strain or Cell Line
C57/BL6J
Source
Jackson Labs
Stock Number
JAX: 000664
Age at Injection
9-12 weeks
Time After Injection
6-8 weeks
Detection Method
Direct fluorescence
Assay & Results
CA1 region of dorsal hippocampus/pyramidal neurons.
Functional Testing
Tool operated as expected. To activate receptors, we used DREADD Agonist 21-Dihydrochloride and CNO.