pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] in Rat (AAV5)
Addgene #62723-AAV5
- Data Submitted By
- Benjamin Saunders
- Submission Date
- July 16, 2019
- Publication Date
- July 16, 2019 (modified May 16, 2023)
- Abstract
- Not Provided
Image attribution: Amy Wolff, PhD
Any further use of this image requires permission from the lab who submitted the data.
- Vector Used
-
pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato]
Addgene #62723-AAV5
Virus & Injection
Virus
- Virus Name
- pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato]
- Serotype
- AAV5
- Source
- Addgene
- Catalog Number
- 62723-AAV5
- Promoter
- Synapsin
- Cargo Type
- Optogenetics
- Injection Titer / Dose
- 1.00E+13 GC/ml
- Injection Volume
- 750 nL
- Injection Rate
- 100 nL/min
- Injector Material
- Not Provided
- Injection Site / Route
- Ventral tegmental area, NeuroNames ID: 521
- Additional Details
- Targeting dopamine neurons in tyrosine-hydroxylase cre rats. Injections targeted to the ventral tegmental area and substantia nigra.
Other Details
- Species
- Rat
- Strain or Cell Line
- Long Evans
- Source
- Not provided
- Age at Injection
- ~4 months
- Time After Injection
- 5 weeks
- Detection Method
- Immunohistochemistry, Direct fluorescence
- Assay & Results
- Dopaminergic/TH+ cells, as expected. The genetic targeting worked as expected.