pAAV-hSyn-DIO-hM4D(Gi)-mCherry in Rat (AAV2)
Addgene #44362-AAV2
- Data Submitted By
- Christina Ruiz and Stephen Mahler
- Submission Date
- June 25, 2019
- Publication Date
- June 25, 2019 (modified May 16, 2023)
- Abstract
- Not Provided
Image attribution: Erik Castillo and Mitchell Farrell
Any further use of this image requires permission from the lab who submitted the data.
- Vector Used
-
pAAV-hSyn-DIO-hM4D(Gi)-mCherry
Addgene #44362-AAV2
Virus & Injection
Virus
- Virus Name
- pAAV-hSyn-DIO-hM4D(Gi)-mCherry
- Serotype
- AAV2
- Source
- Addgene
- Catalog Number
- 44362-AAV2
- Promoter
- Synapsin
- Cargo Type
- Chemogenetics
- Injection Titer / Dose
- 5.00E+12 GC/ml
- Injection Volume
- 300 nl
- Injection Rate
- 300 nl/min
- Injector Material
- Not Provided
- Injection Time
- 7 min.
- Injection Site / Route
- Ventral Pallidum, NeuroNames ID: 1605
- Additional Details
- Targeting GABA neurons in Ventral Pallidum.
Other Details
- Species
- Rat
- Strain or Cell Line
- LE-Tg(Gad1-iCre)3Ottc
- Source
- Rat Resource & Research Center
- Stock Number
- 751
- Age at Injection
- postnatal day 60-90
- Time After Injection
- 3 weeks
- Detection Method
- Immunofluorescence staining for mCherry and Substance P
- Assay & Results
- This virus had dorsal spread in GADiCre transgenic rats if the pipette was not removed very slowly after injection. We also waited 7 min before removing the pipette.
- Other Information
- No toxicity or inflammation was seen
- Tips & Comments
- We also waited 7 min before removing the pipette