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pAAV-hSyn-hM3D(Gq)-mCherry in Rat (AAV Retrograde)

Addgene #50474-AAVrg

Data Submitted By
David Bortz and Anthony A Grace
Submission Date
July 09, 2019
Publication Date
July 09, 2019 (modified May 16, 2023)
Abstract
Not Provided
Caption: This is the endogenous mCherry reporter showing good viral expression in the orbitofrontal cortex 12 weeks after infusion into the medial septum.

Any further use of this image requires permission from the lab who submitted the data.

Vector Used
pAAV-hSyn-hM3D(Gq)-mCherry
Addgene #50474-AAVrg

Virus & Injection

Virus

Virus Name
pAAV-hSyn-hM3D(Gq)-mCherry
Serotype
AAV Retrograde
Source
Addgene
Catalog Number
50474-AAVrg
Promoter
Synapsin
Cargo Type
Chemogenetics
Injection Titer / Dose
7.00E+12 GC/ml
Injection Volume
8 μL- 1 injection
Injection Rate
0.1 μl/min
Injector Material
Not Provided
Injection Site / Route
Medial septal nucleus, NeuroNames ID: 262
Additional Details
Non-specific cell type. We tried to inject exclusively into the medial septum and avoid lateral septum or the horizontal limb of the diagonal band.
View in Addgene Catalog

Other Details

Species
Rat
Strain or Cell Line
Sprague-Dawley
Source
Envigo
Age at Injection
Young adult (weight 300-325 grams)
Time After Injection
12 weeks
Detection Method
Immunohistochemistry
Assay & Results
Expression was found throughout the brain in cell bodies of common afferents to the medial septum. These included the ventral tegmental area, lateral septum, and medial and orbital prefrontal cortices. The expression was not cell-type specific as far as we could tell
Functional Testing
These studies were preliminary studies to determine viral location and spread. No CNO has been given yet so we do not know the answer to this question.