pAAV-hSyn-hM3D(Gq)-mCherry in Rat (AAV Retrograde)
Addgene #50474-AAVrg
- Data Submitted By
- David Bortz and Anthony A Grace
- Submission Date
- July 09, 2019
- Publication Date
- July 09, 2019 (modified May 16, 2023)
- Abstract
- Not Provided
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- Vector Used
-
pAAV-hSyn-hM3D(Gq)-mCherry
Addgene #50474-AAVrg
Virus & Injection
Virus
- Virus Name
- pAAV-hSyn-hM3D(Gq)-mCherry
- Serotype
- AAV Retrograde
- Source
- Addgene
- Catalog Number
- 50474-AAVrg
- Promoter
- Synapsin
- Cargo Type
- Chemogenetics
- Injection Titer / Dose
- 7.00E+12 GC/ml
- Injection Volume
- 8 μL- 1 injection
- Injection Rate
- 0.1 μl/min
- Injector Material
- Not Provided
- Injection Site / Route
- Medial septal nucleus, NeuroNames ID: 262
- Additional Details
- Non-specific cell type. We tried to inject exclusively into the medial septum and avoid lateral septum or the horizontal limb of the diagonal band.
Other Details
- Species
- Rat
- Strain or Cell Line
- Sprague-Dawley
- Source
- Envigo
- Age at Injection
- Young adult (weight 300-325 grams)
- Time After Injection
- 12 weeks
- Detection Method
- Immunohistochemistry
- Assay & Results
- Expression was found throughout the brain in cell bodies of common afferents to the medial septum. These included the ventral tegmental area, lateral septum, and medial and orbital prefrontal cortices. The expression was not cell-type specific as far as we could tell
- Functional Testing
- These studies were preliminary studies to determine viral location and spread. No CNO has been given yet so we do not know the answer to this question.