pAAV-CaMKIIa-hChR2(H134R)-EYFP in Mouse (AAV9)
Addgene #26969-AAV9
- Data Submitted By
- Kyle Puhger and Brian Wiltgen
- Submission Date
- June 19, 2019
- Publication Date
- June 19, 2019 (modified May 16, 2023)
- Abstract
- Not Provided
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Image attribution: Kyle Puhger
Image attribution: Kyle Puhger
Any further use of this image requires permission from the lab who submitted the data.
- Vector Used
-
pAAV-CaMKIIa-hChR2(H134R)-EYFP
Addgene #26969-AAV9
Virus & Injection
Virus
- Virus Name
- pAAV-CaMKIIa-hChR2(H134R)-EYFP
- Serotype
- AAV9
- Source
- Addgene
- Catalog Number
- 26969-AAV9
- Promoter
- CaMKII
- Cargo Type
- Optogenetics
- Injection Titer / Dose
- 1.50E+13 GC/mL
- Injection Volume
- 250 nL
- Injection Rate
- 120 nL/min
- Injector Material
- Not Provided
- Injection Site / Route
- CA1 field, NeuroNames ID: 185
- Additional Details
- Viral injections were targeted to the dorsal CA1 subregion of the hippocampus.
Other Details
- Species
- Mouse
- Strain or Cell Line
- C57/BL6J
- Source
- Jackson Labs
- Stock Number
- JAX: 000664
- Age at Injection
- 10-14 weeks
- Time After Injection
- 2 weeks
- Detection Method
- Immunohistochemistry
- Assay & Results
- Putative CaMKII+ cells in the pyramidal layer of the CA1 subregion.
- Functional Testing
- 473nm blue light was used to activate the ChR2 opsin.