pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA in Mouse (AAV9)
Addgene #20297-AAV9
- Data Submitted By
- Michael Chiang and Sarah E. Ross
- Submission Date
- July 03, 2019
- Publication Date
- July 03, 2019 (modified May 16, 2023)
- Abstract
- Not Provided
Image attribution: null
Any further use of this image requires permission from the lab who submitted the data.
- Vector Used
-
pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA
Addgene #20297-AAV9
Virus & Injection
Virus
- Virus Name
- pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA
- Serotype
- AAV9
- Source
- Addgene
- Catalog Number
- 20297-AAV9
- Promoter
- EF1a
- Cargo Type
- Optogenetics
- Injection Titer / Dose
- 2.88E+13 GC/mL
- Injection Volume
- 500 nL
- Injection Rate
- 100 nL/min
- Injector Material
- Not Provided
- Injection Site / Route
- Lateral parabrachial nucleus, NeuroNames ID: 588
- Additional Details
- Crh-cre population expresses the corticotropin releasing hormone intended to target lateral parabrachial nucleus.
Other Details
- Species
- Mouse
- Strain or Cell Line
- B6(Cg)-Crhtm1(cre)Zjh/J
- Source
- Jackson Labs
- Stock Number
- 12704
- Age at Injection
- 8 weeks
- Time After Injection
- 4 weeks
- Detection Method
- Immunohistochemistry
- Assay & Results
- Although the injection targeted lateral parabrachial nucleus, we observed expression in the adjacent region Kolliker Fuse (KF). We have not confirmed whether these neurons are excitatory or inhibitory. We observed expression of virus in these the Crh-cre line. However, we have not confirmed whether these are indeed expressing Crh.
- Other Information
- No obvious excitotoxicity was observed - the cell bodies did not exhibit any blebbing.
- Tips & Comments
- Perhaps reduction in virus volume (but the expression appeared localized).