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AAV.CamKII.GCaMP6s.WPRE.SV40 in Mouse (AAV9)

Addgene #107790-AAV9

Data Submitted By
Michael Sokoletsky and Ilan Lampl
Submission Date
July 21, 2019
Publication Date
July 21, 2019 (modified May 16, 2023)
Abstract
Not Provided
Caption: Images in two-photon microscope with galvometric scanning (~0.5 Hz), field of view 0.5 x 0.5 mm, super 20x objective with NA = 0.8, green channel (alkali PMT).

Any further use of this image requires permission from the lab who submitted the data.

Vector Used
AAV.CamKII.GCaMP6s.WPRE.SV40
Addgene #107790-AAV9

Virus & Injection

Virus

Virus Name
AAV.CamKII.GCaMP6s.WPRE.SV40
Serotype
AAV9
Source
Addgene
Catalog Number
107790-AAV9
Promoter
CaMKII
Cargo Type
Biosensor
Injection Titer / Dose
2.00E+11 GC/ml
Injection Volume
500 nl
Injection Rate
30 nl/min to prevent backflow
Injector Material
Not Provided
Injection Site / Route
Whisker sensory area (Barrel Cortex) NeuroNames ID : 2703
Additional Details
I was targeting all excitatory cells in the barrel cortex; Forebrain: Cerebral cortex (neocortex); Expression was local to the barrel cortex. Assumed excitatory cells, but we did not attempt to verify co-localization between the virus expression and CamKII as these experiments were largely used for testing
View in Addgene Catalog

Other Details

Species
Mouse
Strain or Cell Line
C57/BL6J
Source
Not provided
Age at Injection
6-10 weeks
Time After Injection
2 weeks
Detection Method
Immunohistochemistry, Direct fluorescence
Assay & Results
Expression was local to the barrel cortex. Assumed excitatory cells, but we did not attempt to verify co-localization between the virus expression and CamKII as these experiments were largely used for testing.
Other Information
Injection was at multiple sites (-300 um, -450 um, and -600 um) below the dura to prevent backflow but also spread the virus more equally