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pAAV-hSyn-DIO-hM3D(Gq)-mCherry in Rat (AAV2)

Addgene #44361-AAV2

Data Submitted By: Todd Nentwig and L. Judson Chandler
Submission Date: December 19, 2020
Publication Date: December 19, 2020 (modified May 16, 2023)
Abstract: Not provided

Sparse labeling of Thalamic projecting Insula neurons using a retrograde Cre + local DIO viral approach. — Caption: Sparse labeling of Thalamic projecting Insula neurons using a retrograde Cre + local DIO viral approach.

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Vector Used: pAAV-hSyn-DIO-hM3D(Gq)-mCherry
Addgene #44361-AAV2

Virus & Injection

Virus

Virus Name: pAAV-hSyn-DIO-hM3D(Gq)-mCherry
Serotype: AAV2
Source: Addgene
Catalog Number: 44361-AAV2
Promoter: Synapsin
Cargo Type: Chemogenetics
Injection Titer / Dose: 6.00E+12 GC/mL
Injection Volume: 500 nL
Injection Rate: Not provided
Injector Material: Not provided
Injection Site / Route: Insular Cortex, NeuroNames: 3613

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Other Details

Species: Rat
Strain or Cell Line: Long-Evans
Source: Not provided
Age at Injection: ~100 days old
Time After Injection: 6 weeks
Detection Method: Immunohistochemistry
Assay & Results: Sparse labeling of Thalamic projecting Insula neurons using a retrograde Cre + local DIO viral approach.