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pAAV.GfaABC1D.PI.Lck-GFP.SV40 in Rat (AAV5)

Addgene #105598-AAV5

Data Submitted By: Todd Nentwig and L. Judson Chandler
Submission Date: January 05, 2020
Publication Date: January 05, 2020 (modified May 16, 2023)
Abstract: Not provided

Rat Lateral Habenula; 10x mag. Astrocytes labeled using GfaABC1D.PI.Lck-GFP virus (green). Synapsin and Glutamate Transporter 1 (GLT1) are labeled with red and cyan staining, respectively. — Caption: Rat Lateral Habenula; 10x mag. Astrocytes labeled using GfaABC1D.PI.Lck-GFP virus (green). Synapsin and Glutamate Transporter 1 (GLT1) are labeled with red and cyan staining, respectively.
Image attribution: Anna Kruyer

Any further use of this image requires permission from the lab who submitted the data.

Vector Used: pAAV.GfaABC1D.PI.Lck-GFP.SV40
Addgene #105598-AAV5

Virus & Injection

Virus

Virus Name: pAAV.GfaABC1D.PI.Lck-GFP.SV40
Serotype: AAV5
Source: Addgene
Catalog Number: 105598-AAV5
Promoter: GFAP
Cargo Type: Control
Injection Titer / Dose: 7.00E+12 GC/ml
Injection Volume: 200 nl
Injection Rate: 1 nl/sec
Injector Material: Not provided
Injection Site / Route: Lateral Habenula, NeuroNames ID: 295
Additional Details: Lateral habenula; Lateral Habenula Astrocytes

View in Addgene Catalog

Other Details

Species: Rat
Strain or Cell Line: Sprague-Dawley
Age at Injection: ~150 days
Time After Injection: 4 weeks
Detection Method: Direct fluorescence
Tips & Comments: Direct fluorescence was sufficient to visualize site/spread of virus expression but immuno-amplification is likely necessary if performing high magnification, quantitative analysis of astrocyte-neuron interactions