pENN.AAV.hSyn.Cre.WPRE.hGH in Mouse (AAV1)
Addgene #105553-AAV1
- Data Submitted By
- Farhan Ali and Alex Kwan
- Submission Date
- July 10, 2019
- Publication Date
- July 10, 2019 (modified May 16, 2023)
- Abstract
- Not provided
Any further use of this image requires permission from the lab who submitted the data.
- Vectors Used
-
pENN.AAV.hSyn.Cre.WPRE.hGH
Addgene #105553-AAV1 -
pAAV-hSyn1-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA
Addgene #68720-AAV1
Virus & Injection
Virus 1
- Virus Name
- pENN.AAV.hSyn.Cre.WPRE.hGH
- Serotype
- AAV1
- Source
- Addgene
- Catalog Number
- 105553-AAV1
- Promoter
- Synapsin
- Cargo Type
- Recombinase
- Injection Titer / Dose
- 1.00E+08 GC/ml
- Injection Volume
- 200 nl
- Injection Rate
- 40 nl/min
- Injector Material
- Not provided
- Injection Site / Route
- Dorsal anterior cingulate cortex/medial secondary motor cortex of mouse. NeuroNames ID : 1062
- Additional Details
- Injection site: dorsal anterior cingulate cortex/medial secondary motor cortex of mouse Cell type: any neuron; Forebrain: Cerebral cortex (neocortex); Dorsal anterior cingulate cortex/medial secondary motor cortex of mouse. Neuronal cell class
Virus 2
- Virus Name
- pAAV-hSyn1-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA
- Serotype
- AAV1
- Source
- Addgene
- Catalog Number
- 68720-AAV1
- Promoter
- Synapsin
- Injection Titer / Dose
- 1.00E+13 GC/ml
- Injection Volume
- 200 nl
- Injection Rate
- 40 nl/min
- Injector Material
- Not provided
- Injection Site / Route
- Dorsal anterior cingulate cortex/medial secondary motor cortex of mouse. NeuroNames ID : 1062
- Additional Details
- Injection site: dorsal anterior cingulate cortex/medial secondary motor cortex of mouse. Cell type: any neuron type; Forebrain: Cerebral cortex (neocortex)
Other Details
- Species
- Mouse
- Strain or Cell Line
- C57/BL6J
- Source
- Not provided
- Age at Injection
- 8-20 weeks
- Time After Injection
- 5 weeks
- Detection Method
- Direct fluorescence
- Assay & Results
- Not provided
- Other Information
- This experiment targeted very sparse expression of GCaMP6s by combining Cre-dependent GCaMP6s expression with injection of very low titer of virus carrying Cre recombinase