pAAV.Syn.Flex.GCaMP6f.WPRE.SV40 in Mouse (AAV1)
Addgene #100833-AAV1
- Data Submitted By
- Bo Li and Xiong Xiao
- Submission Date
- July 09, 2019
- Publication Date
- July 09, 2019 (modified May 16, 2023)
- Abstract
- Not Provided
Any further use of this image requires permission from the lab who submitted the data. This scientist responded to a specific request for user data and was offered compensation for time and input.
- Vector Used
-
pAAV.Syn.Flex.GCaMP6f.WPRE.SV40
#100833-AAV1
Virus & Injection
Virus
- Virus Name
- pAAV.Syn.Flex.GCaMP6f.WPRE.SV40
- Serotype
- AAV1
- Catalog Number
- 100833-AAV1
- Promoter
- Synapsin
- Cargo Type
- Biosensor
- Injection Titer / Dose
- 5.00E+12 GC/ml
- Injection Volume
- 300 nl
- Injection Rate
- 30 nL/min
- Injector Material
- Not Provided
- Injection Site / Route
- Insula cortex, NeuroNames ID: 111
- Additional Details
- Insular cortex and Fezf2 neurons in layer 5/6.
Other Details
- Species
- Mouse
- Strain or Cell Line
- Fezf2-creER
- Source
- Not provided
- Age at Injection
- 8 weeks
- Time After Injection
- 6 weeks
- Detection Method
- Immunohistochemistry, in vivo calcium imaging
- Assay & Results
- Not Provided.
- Functional Testing
- Tamoxifen was prepared by dissolving in corn oil (20 mg/ml) at room temperature with constant rotation overnight. After virus injection, mice were administered with tamoxifen injection under anaesthesia. The same dose (0. 1mg per gram body weight) was administrated by intraperitoneal injection in total three times (day 2, day 5 and day 8 after virus injection)
- Other Information
- After long-term expression, there is some toxicity
- Tips & Comments
- Have a test of different dilutions of this virus for a specific brain region.