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pAAV.Syn.Flex.GCaMP6f.WPRE.SV40 in Mouse (AAV1)

Addgene #100833-AAV1

Data Submitted By
Xiong Xiao and Bo Li
Submission Date
July 09, 2019
Publication Date
July 09, 2019 (modified May 16, 2023)
Abstract
Not Provided

Any further use of this image requires permission from the lab who submitted the data.

Vector Used
pAAV.Syn.Flex.GCaMP6f.WPRE.SV40
Addgene #100833-AAV1

Virus & Injection

Virus

Virus Name
pAAV.Syn.Flex.GCaMP6f.WPRE.SV40
Serotype
AAV1
Source
Addgene
Catalog Number
100833-AAV1
Promoter
Synapsin
Cargo Type
Biosensor
Injection Titer / Dose
5.00E+12 GC/ml
Injection Volume
300 nl
Injection Rate
30 nL/min
Injector Material
Not Provided
Injection Site / Route
Insula cortex, NeuroNames ID: 111
Additional Details
Insular cortex and Fezf2 neurons in layer 5/6.
View in Addgene Catalog

Other Details

Species
Mouse
Strain or Cell Line
Fezf2-creER
Source
Not provided
Age at Injection
8 weeks
Time After Injection
6 weeks
Detection Method
Immunohistochemistry, in vivo calcium imaging
Assay & Results
Not Provided.
Functional Testing
Tamoxifen was prepared by dissolving in corn oil (20 mg/ml) at room temperature with constant rotation overnight. After virus injection, mice were administered with tamoxifen injection under anaesthesia. The same dose (0. 1mg per gram body weight) was administrated by intraperitoneal injection in total three times (day 2, day 5 and day 8 after virus injection)
Other Information
After long-term expression, there is some toxicity
Tips & Comments
Have a test of different dilutions of this virus for a specific brain region.