pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA in Mouse (AAV9)
Addgene #20298-AAV9
- Data Submitted By
- Gaqi Tu and Kaori Takehara-Nishiuchi
- Submission Date
- June 23, 2019
- Publication Date
- June 23, 2019 (modified May 16, 2023)
- Abstract
- Not Provided
Any further use of this image requires permission from the lab who submitted the data.
- Vector Used
-
pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA
Addgene #20298-AAV9
Virus & Injection
Virus
- Virus Name
- pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA
- Serotype
- AAV9
- Source
- Addgene
- Catalog Number
- 20298-AAV9
- Promoter
- EF1a
- Cargo Type
- Optogenetics
- Injection Titer / Dose
- 2.20E+13 GC/mL
- Injection Volume
- 600-800 nL
- Injection Rate
- 200 nL/min for 100 nL and 50 nL/min for 500~700 nL
- Injector Material
- Not Provided
- Injection Site / Route
- Basal forebrain, NeuroNames ID: 1997
Other Details
- Species
- Mouse
- Strain or Cell Line
- B6J.129S6-Chat/MwarJ
- Source
- Jackson Labs
- Stock Number
- 28861
- Other Information
- Genotype: Homozygous for Chat.
- Age at Injection
- 8-12 weeks
- Time After Injection
- 6 weeks
- Detection Method
- Immunohistochemistry
- Assay & Results
- Basal forebrain region. ChAT+ cholinergic neurons and non-cholienrgic neurons (less than 2% among virus expressed cells).
- Functional Testing
- The blue laser was used to activate the terminal of the cholinergic neurons which express the ChR2.
- Tips & Comments
- Wait ~20min after injection.