pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA in Mouse (AAV9)
Addgene #20298-AAV9
- Data Submitted By
- Avishek Adhikari
- Submission Date
- July 18, 2019
- Publication Date
- July 18, 2019 (modified May 16, 2023)
- Abstract
- Not Provided
Any further use of this image requires permission from the lab who submitted the data.
- Vector Used
-
pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA
Addgene #20298-AAV9
Virus & Injection
Virus
- Virus Name
- pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA
- Serotype
- AAV9
- Source
- Addgene
- Catalog Number
- 20298-AAV9
- Promoter
- EF1a
- Cargo Type
- Optogenetics
- Injection Titer / Dose
- 2.00E+12 GC/ml
- Injection Volume
- 60 nl
- Injection Rate
- 300 nL/min
- Injector Material
- Not Provided
- Injection Site / Route
- Hypothalamus NeuroNames ID : 375
- Additional Details
- Cholecystokinin (cck) cells in the hypothalamus of a cck-cre mouse line; Forebrain: Hypothalamus; Cholecystokinin (cck) cells in the hypothalamus of a cck-cre mouse line
Other Details
- Species
- Mouse
- Strain or Cell Line
- C57/BL6J
- Source
- Jackson Labs
- Stock Number
- JAX: 000664
- Age at Injection
- 2 months
- Time After Injection
- 7 weeks
- Detection Method
- Direct fluorescence
- Assay & Results
- Not Provided
- Functional Testing
- Chr2 was activated with blue light and the behavior worked