pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA in Mouse (AAV5)
Addgene #20298-AAV5
- Data Submitted By
- Kengo Inada and Kazunari Miyamichi
- Submission Date
- July 31, 2019
- Publication Date
- July 31, 2019 (modified May 16, 2023)
- Abstract
- Not Provided
Any further use of this image requires permission from the lab who submitted the data.
- Vectors Used
-
pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA
Addgene #20298-AAV5 -
pENN.AAV.hSyn.Cre.hGH
Addgene #105555-AAV9
Virus & Injection
Virus 1
- Virus Name
- pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA
- Serotype
- AAV5
- Source
- Addgene
- Catalog Number
- 20298-AAV5
- Promoter
- EF1a
- Cargo Type
- Optogenetics
- Injection Titer / Dose
- 7.70E+12 GC/ml
- Injection Volume
- 200 nl
- Injection Rate
- 50 nl/min
- Injector Material
- Not Provided
- Injection Site / Route
- Primary motor cortex, Neuronames ID: 1910
- Additional Details
- Primary motor cortex.
Virus 2
- Virus Name
- pENN.AAV.hSyn.Cre.hGH
- Serotype
- AAV9
- Source
- Addgene
- Catalog Number
- 105555-AAV9
- Promoter
- Synapsin
- Cargo Type
- Recombinase
- Injection Titer / Dose
- 2.30E+13 GC/ml
- Injection Volume
- 200 nl
- Injection Rate
- 50 nl/min
- Injector Material
- Not Provided
- Injection Site / Route
- Primary motor cortex, Neuronames ID: 1910
- Additional Details
- Primary motor cortex.
Other Details
- Species
- Mouse
- Strain or Cell Line
- C57/BL6J
- Source
- Not provided
- Age at Injection
- 6 weeks old
- Time After Injection
- 2 weeks
- Detection Method
- Immunohistochemistry, Direct fluorescence
- Assay & Results
- In situ hybridization was performed to enhance YFP signal.
- Functional Testing
- Yes